Liquid Chromatography Tandem Mass Spectrometry Service
- Identification and quantification of drug metabolites.
- Monitoring of in vivo drug dynamics (pharmacokinetic studies).
- Clinical drug concentration testing to support personalized medicine.
- Detection of biomarkers, such as cancer markers and cardiovascular disease markers.
- Quantification of hormone levels, including glucocorticoids and sex hormones.
- Detection of toxins in blood and urine to support clinical diagnostics.
- Detection of pesticide and veterinary drug residues in food products.
- Quantitative analysis of food additives and flavor compounds.
- Analysis of heavy metals, contaminants, and bacterial toxins in food.
- Analysis of organic pollutants (e.g., pesticides, industrial chemicals) in water and soil.
- Detection of volatile organic compounds (VOCs) and persistent organic pollutants (POPs) in air.
- Comprehensive analysis of metabolites in biological samples.
- Construction of metabolic pathways and monitoring of dynamic changes in metabolism.
- Analysis of protein structures and modifications (e.g., phosphorylation, glycosylation).
- Investigation of protein-protein interactions and functional studies.
Liquid chromatography tandem mass spectrometry is a highly sensitive analytical technique that combines liquid chromatography for efficient separation with mass spectrometry for precise quantification. It is extensively utilized for qualitative and quantitative analysis of complex biological samples. With exceptional separation efficiency and precision, liquid chromatography tandem mass spectrometry has applications across biopharmaceuticals, environmental monitoring, food safety, and clinical diagnostics.
As a leading provider of laboratory services, MtoZ Biolabs offers high-quality liquid chromatography tandem mass spectrometry service to deliver accurate and reliable results for complex sample analyses. Utilizing state-of-the-art instrumentation and rigorous operational standards, we ensure precise and efficient analyses tailored to client needs.
Technical Principles
Liquid chromatography tandem mass spectrometry technology combines the efficient separation capabilities of liquid chromatography with the high-sensitivity detection capabilities of mass spectrometry, allowing for the rapid and precise identification and quantification of target components in complex samples.
The liquid chromatography system first separates the sample components based on their chemical interactions with the stationary phase of the chromatographic column. Reverse-phase chromatography, a commonly used technique, achieves efficient separation by modulating the polarity of the mobile phase. This approach is particularly effective for analyzing small molecules, peptides, and other biological samples. Regardless of the complexity of the biological matrix or the diversity of sample components, reverse-phase chromatography ensures reliable and precise separation results.
Following separation, sample components are subjected to mass spectrometric analysis. At this stage, the sample molecules are ionized into charged ions by an ion source and subsequently analyzed by a mass analyzer. Commonly used mass spectrometers include triple quadrupole (QqQ) and high-resolution Orbitrap systems. The QqQ system employs two quadrupoles and a collision cell to select precursor ions, induce collision dissociation, and produce fragment ions, thereby providing detailed structural information about the target compounds. In contrast, Orbitrap mass spectrometers deliver even higher resolution and precision, making them ideal for in-depth analysis of complex sample compositions.
Thomas, S. N. et al. Nat Rev Methods Primers. 2022.
Analysis Workflow
To ensure high-quality results in every liquid chromatography tandem mass spectrometry service, MtoZ Biolabs adheres to standardized procedures and offers customized services tailored to client-specific needs.
1. Sample Receipt and Preliminary Inspection
Upon receiving samples, MtoZ Biolabs inspects their integrity and suitability. We recommend appropriate pretreatment protocols for each sample type (e.g., serum, urine, tissue) to ensure optimal analysis.
2. Sample Pretreatment
Based on sample characteristics, we apply methods such as extraction, concentration, and purification to remove contaminants, ensuring accurate and precise results for subsequent analysis.
3. Liquid Chromatography Separation
Pretreated samples are processed through liquid chromatography, where target compounds are separated from the matrix. We optimize separation conditions for high-resolution, reproducible results.
4. Mass Spectrometry Analysis
The separated compounds are analyzed using mass spectrometry. Ionized molecules are measured for qualitative and quantitative analysis, providing structural data through advanced techniques.
5. Data Analysis and Reporting
Our team processes mass spectrometry data to generate comprehensive, customized reports, offering reliable results to support informed decision-making in research or quality control.
Franciosa, G. et al. Semin Immunopathol. 2023.
Why Choose MtoZ Biolabs?
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced liquid chromatography tandem mass spectrometry analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all liquid chromatography tandem mass spectrometry analysis data providing clients with a comprehensive data report.
4. Multi-Sample Compatibility: Our platform supports a wide variety of biological samples, including cells, tissues, blood, and bodily fluids, providing flexibility to accommodate diverse research demands.
5. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.
Applications
Liquid chromatography tandem mass spectrometry technology is widely used across various fields for its powerful separation and detection capabilities. MtoZ Biolabs’ liquid chromatography tandem mass spectrometry service leverages this advanced technology to provide precise analytical support for the following applications:
1. Drug Metabolism Research
2. Clinical Diagnostics and Monitoring
3. Food Safety Testing
4. Environmental Monitoring
5. Metabolomics Research
6. Proteomics and Molecular Biology Research
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
With a robust technical platform and a highly experienced team, MtoZ Biolabs’ liquid chromatography tandem mass spectrometry service provides clients with accurate, high-quality analytical data to meet the demands of various research and industrial applications. Whether for drug development, clinical testing, food safety, or environmental monitoring, liquid chromatography tandem mass spectrometry serves as a powerful analytical tool. For consultation and inquiries, please contact our technical team.
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