MALDI Mass Specrometry Analysis Service

The Matrix-Assisted Laser Desorption/Ionization (MALDI) mass spectrometry analysis service uses laser energy absorbed by a matrix to generate ions from macromolecules with minimal fragmentation, allowing for the measurement of the molecular weight of biomolecules. MALDI mass spectrometry primarily utilizes a MALDI ion source and a Time-of-Flight (TOF) mass analyzer, forming the MALDI-TOF mass spectrometer.The principle of the MALDI mass spectrometry analysis service involves spotting various microbial samples with an excess of matrix solution onto a sample plate. After the solvent evaporates, a co-crystal of the sample and matrix is formed. Laser energy is then used to irradiate the crystal. The matrix absorbs energy from the laser, which causes the sample to adsorb. A charge transfer occurs between the matrix and the sample, leading to the ionization of the sample molecules. The sample ions acquire the same kinetic energy in the accelerating electric field. After high-pressure acceleration and focusing, they enter the TOF mass spectrometer for mass analysis. The detector detects ions with different mass-to-charge ratios (m/z), and a pathogen protein fingerprint is generated by plotting m/z ratios on the x-axis and ion peaks on the y-axis. This fingerprint is then compared with a database for identification.Based on mass spectrometry technology combined with quantitative analysis and pathway analysis, MtoZ Biolabs offers MALDI mass spectrometry analysis service that can perform protein analysis, metabolomics analysis, lipidomics analysis, and a variety of other analyses.

 



Hou, C. et al. J Food Drug Anal.2019.

Figure 1. Principle of MALDI-TOF.

 

Technical Advantages of MALDI Mass Spectrometry:

1. MALDI-TOF MS generates singly charged ions, making data interpretation easier.

2. MALDI-TOF MS analysis does not require chromatographic separation.

3. Sample preparation is simple.

 

Services at MtoZ Biolabs

MtoZ Biolabs is equipped with the best-performing desktop MALDI-TOF mass spectrometer. Featuring a gridless reflector and the microflex LRF, it offers exceptional resolution, excellent mass accuracy, and remarkable sensitivity, making it unparalleled by other instruments on the market. The MALDI mass spectrometry analysis service offered by MtoZ Biolabs include:

 

1. MALDI-TOF MS Analysis Service

This is an efficient technique used for analyzing biomolecules such as proteins, peptides, and nucleic acids. Through this service, clients can obtain critical information about the molecular weight and structure of their samples.

 

2. Lipidomics MALDI Imaging Service

MtoZ Biolabs also offers lipidomics MALDI imaging services that can be used to detect and locate lipid molecules in biological samples, aiding in the understanding of lipid distribution and function at the cellular and tissue levels.

 

Service Advantages

1. Transparent Pricing

Our pricing is transparent, with no hidden fees or extra charges, offering a high cost-performance ratio.

 

2. Short Service Cycle

MtoZ Biolabs is equipped with the best-performing MALDI-TOF mass spectrometer, ensuring the speed and accuracy of the service. The shortest complete cycle can be as fast as 5 days.

 

3. High-Quality Data

Using more optimized experimental methodologies, we provide high-quality omics data. Through bioinformatics analysis, we deliver comprehensive data interpretation services and complete reports in  English.

 

4. Customized Research Solutions

MtoZ Biolabs offers personalized services tailored to your sample needs, providing one-on-one support through our professional pre- and post-sales teams.

 

Sample Submission Suggestions

We accommodate a variety of sample types for analysis, including:

 

1. Biomolecules

Proteins, peptides, nucleic acids, etc.

 

2. Microbial Identification

Bacteria, fungi, etc.

 

3. Pharmaceutical Analysis

Determining the molecular weight of various drugs.

 

4. Clinical Samples

Analyzing proteins, peptides, and other biomarkers in blood, urine, and other body fluid samples.

*Note: If you have any special requirements or need assistance with sample preparation, please contact us.

 

Applications

1. Clinical Microbiology

Rapid identification of bacteria and fungi, predicting microbial resistance to certain antibiotics.

 

2. Proteomics

Determining the molecular weight and primary structure of proteins, further analyzing protein expression levels under different conditions.

 

3. Biopharmaceuticals

Analyzing the quality and purity of protein-based drugs.

 

4. Biomarker Discovery

Identifying and validating biomarkers.

 

5. Food Safety

Detecting microbial contamination in food, such as Salmonella, E. coli, etc.

 

6. Environmental Science

Identifying microbial species in environmental samples, such as soil, water, and air.

 

7. Drug Metabolite Identification

Studying the metabolic pathways of drugs by analyzing their metabolites in the body.

 

Case Study

1. Deubiquitinase analysis for drug discovery based on high-throughput matrix-assisted laser desorption mass spectrometry (MALDI-TOF)

This article presents a mass spectrometry-based screening method for deubiquitinase (DUB) activity. Using MALDI mass spectrometry, the single ubiquitin signal generated by DUB cleavage of ubiquitin chains is detected to assess DUB enzyme activity. The MALDI mass spectrometry analysis service offers advantages such as high throughput, the use of physiological substrates, and the sensitivity and reliability of mass spectrometry readings. This service can be applied in drug discovery for screening efficient and specific DUB modulators.

 



De Cesare,V. et al. Nat Protoc.2020.

Figure 2. HTS MALDI-TOF DUB Analysis Workflow.

 

Deliverables

1. Comprehensive Experimental Details

 

2. Materials, Instruments, and Methods

 

3. Data Analysis, Preprocessing, and Estimation

 

4. Bioinformatics Analysis

 

5. Raw Data Files

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!