Mass Spectrometry Protein Sequencing Service

Mass Spectrometry Protein Sequencing is an advanced technique for addressing complex protein sequence analysis, capable of accurately identifying and interpreting the amino acid sequences of unknown proteins. Proteins function both inside and outside cells and are involved in nearly all biological processes. As the final executors of gene expression, they directly participate in various intricate activities such as cellular metabolism, signal transduction, and immune responses. Therefore, precise protein sequence analysis is not only central to fundamental biological research but also a critical step in precision medicine, early disease diagnosis, and new drug development. Although traditional protein analysis techniques, such as Western Blot and enzyme-linked immunosorbent assay (ELISA), have played important roles in specific fields, their capacity to identify and characterize unknown proteins in complex samples remains limited.

 



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Principle of Mass Spectrometry

 

With the rapid development of mass spectrometry (MS) technology, Mass Spectrometry Protein Sequencing has become a core tool in proteomics research. As a high-sensitivity, high-resolution technology, mass spectrometry can reveal protein amino acid sequences and structural features by analyzing mass-to-charge ratios (m/z) and fragment information. Consequently, Mass Spectrometry Protein Sequencing Service have been widely applied to proteomics analyses of complex biological samples, providing strong support for disease research, drug development, and biomarker screening.

 

Service at MtoZ Biolabs

The core technology of the Mass Spectrometry Protein Sequencing Service is protein sequence decoding based on mass spectrometry analysis. By measuring the mass-to-charge ratio (m/z) of protein molecules and the fragment ions generated under high-energy collisions, mass spectrometry reveals the molecular structure and sequence of proteins. Relying on high-resolution liquid chromatography–mass spectrometry (LC-MS), tandem mass spectrometry (MS/MS), and other techniques, MtoZ Biolabs’ Mass Spectrometry Protein Sequencing Service can comprehensively sequence proteins in complex samples, providing highly accurate amino acid sequence information. Whether it’s a purified single-protein sample or a complex biological sample, MtoZ Biolabs can offer customized services to help clients obtain the precise data they need.

 



 

Service Advantages

1. High Sensitivity and High Resolution

Our Mass Spectrometry Protein Sequencing Service utilizes state-of-the-art instruments such as Orbitrap and Q-TOF mass spectrometers, enabling precise analysis of protein sequences in complex samples and delivering high-resolution mass data. This allows even low-abundance or trace proteins to be accurately measured, ensuring highly reliable results.

 

2. Diverse Sample Compatibility

This service supports various types of protein samples, including but not limited to single proteins, complexes, cell extracts, and tissue samples. Whether your sample is a simple purified protein or a complex biological mixture, we provide the appropriate pretreatment and analysis solutions to ensure experimental success.

 

3. Automated Data Analysis and Report Generation

Our analytical platform, integrated with advanced software tools, automates the processing of mass spectrometry data and accurately identifies protein sequences and their modifications. The final report provides detailed protein identification information, including amino acid sequence, molecular weight, sequence coverage, and other parameters, making subsequent functional research more convenient.

 

4. Multiple Modification Analyses

In addition to conventional protein sequence analysis, MtoZ Biolabs’ Mass Spectrometry Protein Sequencing Service supports both qualitative and quantitative analyses of post-translational modifications (such as phosphorylation, glycosylation, and ubiquitination), helping customers gain deeper insights into the regulatory mechanisms of protein function.

 

Applications

✅ Protein Identification

✅ Post-Translational Modification Analysis

✅ Protein-Protein Interaction Studies

✅ Disease Biomarker Discovery

✅ Drug Target Identification

 

Sample Submission Suggestions

To ensure the accuracy and reliability of sequencing results, MtoZ Biolabs recommends that clients follow these guidelines when submitting samples:

1. Sample Purity

The protein sample should be as purified as possible to minimize contaminants that may affect mass spectrometry accuracy. If the sample contains complex components, we recommend using specialized pretreatment methods, such as immunoprecipitation or affinity chromatography, for preliminary processing.

 

2. Sample Amount

Our service accommodates various sample quantities, typically requiring a minimum protein concentration of 10 μg per sample. For low-abundance samples, we recommend providing a larger sample amount to ensure sufficient signal for analysis.

 

3. Sample Types

We accept various types of samples, including but not limited to tissue extracts, cell lysates, and purified proteins. For special samples (e.g., those containing high levels of salts or organic solvents), please contact us in advance so that we can offer professional pretreatment advice as needed.

 

MtoZ Biolabs’ Mass Spectrometry Protein Sequencing Service is a state-of-the-art technology platform that integrates high sensitivity, high resolution, and diverse applications, and is widely employed in proteomics, disease research, drug development, and more. With precise analytical capabilities and professional technical support, we help clients achieve breakthroughs in complex protein analyses, providing a solid technical foundation for scientific innovation and clinical applications. If you have any related needs, please feel free to contact us. We will provide a personalized service plan to support your research and application endeavors.