Methionine Cycle Metabolism Analysis
- Advanced mass spectrometry technology with high sensitivity and accuracy
- Comprehensive data analysis supported by a team of experienced analysts
- Strict quality control to ensure reliable results
- Personalized service to meet various research needs
Methionine is a vital sulfur-containing amino acid that plays a crucial role in various biological processes, including protein synthesis, methylation reactions, and the production of essential molecules such as S-adenosylmethionine (SAM). The Methionine Cycle, a central metabolic pathway, is intricately involved in these processes, where methionine is converted to SAM, which subsequently donates methyl groups to various substrates, including DNA, RNA, and proteins. This cycle is tightly regulated and closely linked to the folate cycle and transsulfuration pathway, highlighting its significance in maintaining cellular function and epigenetic regulation.
The Methionine Cycle involves several key enzymes, including methionine adenosyltransferase (MAT), which catalyzes the formation of SAM from methionine and ATP. Following methyl group transfer, SAM is converted into S-adenosylhomocysteine (SAH), which is then hydrolyzed by S-adenosylhomocysteine hydrolase (SAHH) to produce homocysteine. These interlinked reactions ensure a balance of methionine and its derivatives, which are critical for maintaining cellular methylation potential and redox homeostasis.
Figure 1 The Methionine Cycle in T Cells
Studying the Methionine Cycle and its metabolites is essential for understanding a variety of physiological and pathological conditions, including cardiovascular diseases, cancer, and neurodegenerative disorders. Disruptions in this cycle can lead to altered methylation patterns, oxidative stress, and accumulation of toxic intermediates, underscoring the importance of precise and comprehensive metabolic profiling.
At MtoZ Biolabs, we have developed a comprehensive Methionine Cycle Metabolism Analysis service to decipher complexities of the methionine cycle and the related metabolic alterations. Our Methionine Cycle Metabolism Analysis service employs an untargeted metabolomics approach powered by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS). This advanced technology provides high precision, high throughput, and broad-spectrum detection of methionine cycle metabolites, facilitating a detailed understanding of this complex metabolic pathway.
Analysis Workflow
Our analysis workflow includes: Sample Collection, Sample Preparation, LC-MS Analysis, Data Processing and Analysis, Report Generation
Service Advantages
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Methionine Cycle Metabolism Analysis
4. Raw Data
5. Custom Analysis Report
For more information, please feel free to contact us. We look forward to assisting you in your methionine cycle metabolism research.
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