MRM

Multiple Reaction Monitoring (MRM) is a quantitative analysis technique based on tandem mass spectrometry (MS/MS) used for the selective detection and quantification of target analytes in complex biological samples. The fundamental principle involves selecting specific precursor ions and their fragment ions using two mass analyzers in the mass spectrometer, thereby enhancing specificity and sensitivity. Initially, the precursor ion (usually a peptide segment) of the target molecule is selected in the first mass analyzer. This precursor ion is then fragmented in the collision cell, and the resulting specific fragment ion is selected in the second mass analyzer. This process allows for the selective detection of target molecules, reduces background noise, and improves quantification accuracy. MRM is particularly suitable for quantifying proteins or peptides in the following scenarios: (1) Proteins or peptides with known sequences: The sequence information of the target protein or peptide must be known in advance to design specific precursor and fragment ion pairs. (2) Low-abundance proteins in complex samples: The high specificity and sensitivity of MRM make it highly effective for quantifying low-abundance proteins in complex samples such as plasma or tissue homogenates. MRM technology, with its high specificity, sensitivity, and multiplex detection capability, plays an increasingly significant role in proteomics research and clinical applications.

 



Thomas, A. et al. J. Chem. Educ. 2018. 

Figure 1. Principle of MRM

 

MtoZ Biolabs employs Multiple Reaction Monitoring (MRM) for the quantitative analysis of proteins and peptides, offering high specificity and sensitivity to support your scientific research. For inquiries about our services, please contact us.

 

Analysis Workflow

1. Sample Preparation: Extract proteins and digest them to generate peptides.

 

2. Mass Spectrometry Parameter Optimization: Select appropriate precursor and fragment ion pairs and optimize mass spectrometry parameters.

 

3. Sample Analysis: Inject the sample into the liquid chromatography-tandem mass spectrometry system (LC-MS/MS) for separation and detection.

 

4. Data Analysis: Use specialized software to process mass spectrometry data for quantitative analysis and interpretation of results.

 

Applications

1. Quantification of Disease Markers: Accurately quantify disease-related proteins to aid in diagnosis and monitoring.

 

2. Drug Metabolism Research: Analyze proteins and enzymes involved in drug metabolism pathways.

 

3. Biomarker Validation: Validate potential biomarkers in large-scale proteomics research.

 

Service Advantages

• High Specificity and Sensitivity: By selecting specific precursor and fragment ions, detection specificity and sensitivity are improved.
• Multiplex Detection Capability: Simultaneously quantify multiple target proteins, suitable for high-throughput analysis.
• Wide Applicability: Suitable for quantitative analysis in various complex biological samples.