MtoZ Biolabs High-Depth Plasma Proteomics Service (HD Blood Plus)
- Differential Plasma Proteome Analysis: Identifies differentially expressed proteins across samples with high sensitivity
- Tissue-Specific Protein Expression: Detects tissue-specific proteins, providing insights into organ-specific diseases and conditions.
- Drug Interaction Analysis: Explores protein-drug interactions using comprehensive databases such as DrugBank, facilitating drug development and therapeutic research.
Plasma proteomics plays a crucial role in the discovery of biomarkers, disease mechanisms, and therapeutic targets due to the dynamic nature and diversity of plasma proteins. MtoZ Biolabs offers the HD Blood Plus service, which leverages cutting-edge mass spectrometry and data analysis platforms to achieve unprecedented depth and precision in plasma proteome profiling. This service is designed for researchers aiming to explore the complex plasma proteome with high sensitivity and accuracy, particularly in biomarker discovery and clinical research.
Challenges in Plasma Proteomics
1. Extremely wide dynamic range of plasma proteins, spanning from abundant proteins to those present in trace amounts, presents significant analytical challenges. Quantifying low-abundance proteins effectively, among over 10,000+ plasma proteins, requires advanced technologies.
2. The presence of highly abundant proteins such as albumin often masks the detection of low-abundance proteins, limiting the potential for comprehensive analysis.
3. There is a need for greater proteomic coverage in plasma, especially for rare and low-expression proteins that could provide critical insights into disease and treatment responses.
High-Depth Plasma Proteomics Solution
MtoZ Biolabs’ HD Blood Plus service overcomes these challenges through the application of advanced Narrow Window Direct DIA (Data-Independent Acquisition) combined with cutting-edge Orbitrap Astral technology. This solution provides high resolution and sensitivity, enabling detection of low-abundance plasma proteins without the need for depletion of high-abundance proteins. The HD Blood Plus platform ensures comprehensive proteome coverage with optimized depth, giving researchers the ability to capture even the rarest plasma proteins.
Service Advantages
1. Advanced Instrumentation
Utilization of Orbitrap Astral and advanced DIA techniques ensures unmatched sensitivity and depth in plasma proteomics.
2. Comprehensive Project Control
Each project undergoes strict quality control at all stages, from sample preparation to data analysis.
3. Data-Driven Clinical Translation
Clinical relevance is ensured through high-quality data that enables meaningful insights for biomarker identification and drug discovery
4. Protein Detection at 180 SPD (Sample Processing Depth)
Up to 3,000+ unique proteins can be identified.
5. Protein Detection at 60 SPD
The number of detected proteins increases to over 6,000, enabling deep proteome coverage with higher sensitivity and reliability.
Applications
The analysis pipeline leverages comprehensive data sources, such as the Human Plasma Proteome Project (HPPP) and Human Protein Atlas (HPA), for accurate protein quantification, functional annotation, and comparison across various conditions. Advanced data analysis tools, such as π-Hub-Hub Project, are employed to uncover novel insights, functional pathways, and drug interactions, supporting high-impact discoveries.
Sample Delivery Suggestions and Precautions
1. Sample Requirements
Use EDTA anticoagulant blood collection tubes for plasma preparation. After gentle mixing and centrifugation (500g for 10 minutes at 4°C), the supernatant should be aliquoted into 1.5 mL tubes and stored immediately at -80°C.
2. Important Considerations
(1) Avoid hemolysis during sample collection.
(2) Ensure samples are frozen within 3 hours of collection to maintain quality.
(3) The minimum plasma volume required is 100 μL.
How to order?
