MtoZ Biolabs Offers Advanced Chemical Proteomics Analysis Service
Chemical proteomics analysis service integrates synthetic chemistry, cell biology, and mass spectrometry techniques to probe the interactions between small molecules and proteins, aiding in the identification of drug targets and elucidating their mechanisms of action. Widely applied in drug discovery and development, this methodology facilitates the identification of drug targets, optimization of lead compounds, and comprehensive profiling of off-target effects. MtoZ Biolabs offers a one-stop chemical proteomics analysis service powered by high-resolution mass spectrometry. By developing specialized probes and integrating synthetic chemistry with cell biology, we help researchers optimize drug design and improve drug development outcomes.
Workflow and Technologies of MtoZ Biolabs' Chemical Proteomics Analysis Service
We employ a systematic approach in our chemical proteomics analysis, supported by state-of-the-art technologies at each critical step. Below, we detail how our expertise facilitates each stage of your research.
1. Probe Design and Synthesis
The initial step in chemical proteomics analysis service involves the design and synthesis of small molecule probes. These probes must exhibit high selectivity and specificity, enabling them to bind target proteins and form stable complexes. MtoZ Biolabs is a key player in this process, offering four primary types of probes tailored to meet the diverse needs of drug research and target screening:
(1) Immobilized Probes
Bioactive natural products are covalently attached to biocompatible, inert resins (such as agarose or magnetic beads), creating a "bait" for screening active proteomes. These probes efficiently capture target proteins that interact with drug molecules, ensuring high screening efficiency.
(2) Activity-based Probes (ABPs)
Designed based on the activity of drug molecules, ABPs can bind to target proteins before they interact with other cellular proteins. Due to their ability to penetrate cell membranes, ABPs are especially useful for identifying target proteins in living cells, providing robust evidence for the drug’s target specificity and its mechanism of action.
(3) Click Chemistry Probes
These probes utilize bioorthogonal click reactions to form covalent bonds with complementary molecules (such as azides or alkynes). By incorporating affinity tags (e.g., biotin or fluorescent labels), they facilitate subsequent target enrichment and protein identification. Click chemistry probes are effective in identifying target proteins and are ideal for real-time monitoring of drug-protein interactions.
(4) Photoaffinity Probes
Building on the benefits of click chemistry probes, photoaffinity probes include light-activatable groups that generate highly reactive radicals upon exposure to light, enabling covalent binding between the drug molecule and its target protein. This method is particularly valuable for studying drug-target protein interactions in live cells, offering exceptional specificity and sensitivity.
Chen, X. et al. Sig Transduct Target Ther. 2020.
Figure 1. General Molecular Structures of Different Types of Chemical Proteomics Probes
2. Target Capture and Protein Identification
After designing and synthesizing the probes, MtoZ Biolabs employ advanced techniques to ensure highly specific capture and quantitative identification of target proteins, enhancing your research outcomes. The key methodologies include:
(1) Mass Spectrometry (MS)
MtoZ Biolabs applies high-resolution mass spectrometry techniques (such as LC-MS/MS) in chemical proteomics analysis service. By precisely measuring the mass-to-charge ratio of peptides, this method enables the identification of proteins that bind to small molecules. Mass spectrometry not only facilitates qualitative analysis of protein types but also provides quantitative data on protein expression levels and post-translational modifications, offering crucial insights for functional studies of target proteins.
(2) Drug Affinity Responsive Target Stability (DARTS)
DARTS is an ideal method for clients working with natural compounds that are difficult to modify. This label-free technique protects target proteins from proteolytic degradation upon binding to small molecules. By treating cell lysates with your compounds of interest and performing limited proteolysis, MtoZ Biolabs identifies small molecule-protein complexes that resist degradation. This approach provides accurate target identification without altering the compound, offering more precise and reliable data for your research.
(3) Thermal Proteome Profiling (TPP)
TPP evaluates the impact of drug binding on protein stability by analyzing the thermal stability of proteins in cell lysates under varying temperature conditions. Using this technique, MtoZ Biolabs optimizes drug target screening, helping to identify proteins whose stability is altered upon drug binding. This approach provides valuable insights into the specific proteins involved in drug action, aiding the discovery of potential targets for drug development.
3. Data Analysis and Target Deconvolution
Data analysis is a core component of our one-stop chemical proteomics analysis service. We utilize efficient data processing and deconvolution technologies, including our AI-powered bioinformatics platform, to analyze complex experimental data. By delivering actionable insights, we accelerate the discovery of drug mechanisms. Through comprehensive analysis of mass spectrometry data, we precisely identify the direct targets of drugs, uncover key proteins involved in drug interactions, and explore their roles in signaling pathways. Additionally, we investigate the drug-protein interaction network, offering a comprehensive solution for target screening and validation.
Why Choose MtoZ Biolabs?
Our primary advantages include:
1. Advanced Analysis Platform
MtoZ Biolabs' advanced one-stop chemical proteomics analysis platform guarantees reliable, fast, and highly accurate analysis service.
2. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all chemical proteomics analysis service data, providing clients with a comprehensive data report.
What Could Be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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