Mucin Type of O-glycan Analysis Service
- High Sensitivity and Resolution
- Comprehensive Analysis
- Professional Team
- Tailored Service
- Investigating the role of O-glycans in various biological processes
- Studying the interaction between O-glycans and pathogens
- Developing therapeutic strategies targeting O-glycans
- Understanding the mechanism of diseases associated with abnormal O-glycosylation
- Serum/plasma ≥ 500 μl/sample.
- Urine ≥ 1 ml/sample.
- Tissues ≥ 200 mg/sample.
- Cells: ≥ 1 × 107/sample.
- Samples should be stored in a -80℃ environment and transported on dry ice.
Protein glycosylation, a critical post-translational modification, plays an essential role in understanding the varied functions of proteins across physiological and pathological processes. Glycosylation of cell membrane and secreted proteins primarily involves O-type and N-type glycosylation. The biological functions of Mucin-type O-glycans glycosylation, in particular, remain incompletely understood. Mucin-type O-glycans, characterized by the initial attachment of N-acetylgalactosamine (GalNAc) to serine/threonine residues, are the most common type of O-glycosylation in the human body. They play crucial roles in various biological processes, including protein stabilization, cell signaling, and pathogen binding. Thus, exploring its physiological roles in the body and its relationships with diseases represents a key area of focus in advancements in glycomics. At MtoZ Biolabs, we provide comprehensive mucin type of O-glycan analysis service based on liquid chromatography and mass spectrometry (LC-MS/MS) platform to reveal the complexity and diversity of O-glycan structures.
Figure 1. Mucin Type of O-glycan Core Structures And Key Sialoglycoforms
Services at MtoZ Biolabs
At MtoZ Biolabs, our analysis service involves the use of advanced LC-MS/MS platform, which offers high sensitivity and resolution for glycan profiling. We perform comprehensive O-glycan analysis by integrating multiple analytical techniques including glycoprotein digestion, O-glycan release, fluorescent labeling, and LC-MS/MS analysis to ensure high coverage of O-glycans.
Analysis Workflow
1. Sample Collection: We accept various types of samples including tissues, serum, cells, etc.
2. Protein Digestion: Glycoproteins in the sample are digested into peptides.
3. O-Glycan Release: O-glycans are released from peptides by chemical or enzymatic methods.
4. Fluorescent Labeling: The released O-glycans are labeled with a fluorescent tag for detection.
5. LC-MS/MS Analysis: The labeled O-glycans are analyzed by our advanced LC-MS/MS platform.
6. Data Analysis & Report: Detailed analysis report with experimental procedures, MS/MS spectra of glycans, and comprehensive interpretation of the data will be provided.
Service Advantages
Applications
Our mucin type of O-glycan analysis service is suitable for a wide range of applications including but not limited to:
Sample Submission Requirements
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Mucin Type of O-glycan Analysis
4. Mass Spectrometry Images
5. Raw Data
At MtoZ Biolabs, we strive to provide the most reliable and high-quality mucin type of O-glycan analysis service. Contact us today to discuss your project needs.
How to order?
