Bead-Based Multiplex Flow Cytometry Analysis Service

With the widespread application of flow cytometry (FC) and solid-phase technology, along with the revolutionary advancements in microsphere-based multi-parameter detection technology, FC can qualitatively and quantitatively analyze soluble proteins. Unlike traditional ELISA, which is limited to single-parameter detection, FC multiplex detection can analyze multiple parameters simultaneously from a sample. This capability significantly enhances research efficiency and achieve the comprehensive analysis of rare and precious samples. Leading technologies in this field include Luminex, BD's cytometric bead array (CBA) system, and eBioscience's FlowCytomix technology. MtoZ Biolabs employs BD's CBA and eBioscience's FlowCytomix to offer multi-parameter cytokine and other soluble molecule detection services to researchers.

 

The detection principle of this technology mirrors that of sandwich immunoassays. Fluorescent microspheres are coated with antibodies specific to the target antigen. These antibody-coated microspheres are then incubated with the sample, allowing the analytes (e.g., cytokines) to bind to the antibodies on the microspheres. Subsequently, a mixture of biotin-labeled antibodies specifically binds to the captured analytes. Streptavidin-PE is then added to bind to the biotin, enabling fluorescence detection. The flow cytometer differentiates microsphere populations based on size and fluorescence intensity. Two sizes of microspheres (4.4 μm and 5.5 μm) are internally labeled with 9 and 11 different intensities of fluorescent dyes, respectively (far-red fluorescence, excitable by argon, helium-neon, or ultraviolet light sources, with an emission wavelength of 690 nm). This configuration allows the flow cytometer to simultaneously identify and distinguish 20 different microsphere populations, enabling the detection of up to 20 parameters from a single sample.

• • Cardiovascular Diseases Analysis Service

  Cardiovascular diseases are usually associated with systemic inflammatory responses, such as increased expression of inflammatory proteins CRP, IL-1, IL-6, IL-18, and TNF-α. These proteins can be considered as diagnostic markers for the risk assessment of various cardiovascular diseases. For instance, CD40, which belongs to the tumor necrosis factor receptor superfamily, and CD40L play a crucial role in T-cell dependent B-cell responses.

• • Cell Apoptosis Analysis Service

  Cell death occurs through several pathways including apoptosis, necrosis, and other emergent pathways. Apoptosis is pivotal in immune responses, involving processes such as intracellular balance, T cell clustering, the death of reactive lymphocytes, and cytotoxity of target cells. Apoptosis involves key components like APO-1/Fas, a receptor essential for apoptotic signaling; Caspase-3, crucial for apoptosis and osteoclast activation; and CD30, whose activation halts cell growth and triggers apoptosis.......

• • Metabolic Bone Disease Analysis Service

  Metabolic bone disease encompasses a range of bone disorders caused by congenital or acquired factors that disrupt normal bone metabolism and biochemical states, leading to disorders in bone biochemistry and metabolism. These disorders stem from abnormalities in three key areas: bone resorption, bone growth, and mineral deposition. Increasingly, research has identified numerous cytokines involved in the processes of bone remodeling and metabolism.

• • Atopic Dermatitis Analysis Service

  Atopic Dermatitis (AD) is prevalent among children aged 1 to 5 years and a considerable number of patients carry this condition into adulthood. As a chronic inflammatory skin disease, AD often coexists with asthma and rhinitis. At the cellular level, the hallmark of AD is characterized by high serum IgE levels, where T cells play a crucial regulatory role.

• • Tumor Immunology Analysis Service

  During the tumor formation process, cancer cells release various cytokines and growth factors into the surroundings and reprogram many other types of cells to establish a tumor microenvironment. As a result, tumor tissues almost always contain a large number of endothelial cells, fibroblasts, and infiltrating inflammatory cells, which in turn produce various cytokines. Tumor-derived VEGF acts on endothelial cells, promoting angiogenesis and tumor growth, invasion, and metastasis.

• • Systemic Lupus Erythematosus Analysis Service

  Systemic lupus erythematosus (SLE) is a classic systemic autoimmune disorder characterized by impaired autoimmune tolerance and high concentration of autoantibodies targeting self-antigens, which lead to organ damage. This pathological process involves multiple cytokines.

• • Obesity Related Immune Factors Analysis Service

  Obesity is a systemic disease, not only does it lead to changes in body shape, but it also brings along various diseases and complications, affecting an individual's overall health. Common complications, such as hyperglycemia, hyperlipidemia, and hypertension, are medically termed as "metabolic syndrome". The fluid factors secreted by adipose tissue propel the development of metabolic syndrome and vascular diseases.

• • Immune Regulation Related Factors Analysis Service

  To maintain the immune system's homeostasis, multiple control systems are essential for managing infections, inhibiting tumor growth, preventing inflammation, and avoiding autoimmune diseases. T cells are known to be critical in this regulatory process. Specifically, Th3 cells primarily secrete TGF-β along with varying levels of IL-4 and IL-10. Natural regulatory T cells and Tr1 cells can suppress cytokine production in vitro through CD4+/CD25- T cells or antigen-specific Th1 or Th2 cells.

• • Pain Related Immune Factors Analysis Service

  Pain serves as the primary symptom across numerous pathological conditions. Obesity, significantly impacting the nervous system, is recognized as a systemic disease. It not only alters physical appearance but also brings about various diseases and complications, thus deteriorating an individual's overall health. Common complications, including hyperglycemia, hyperlipidemia, and hypertension, are collectively known as "metabolic syndrome."

• • Diabetes Mellitus Analysis Service

  Diabetes is marked by hyperglycemia, resulting from defects in insulin secretion and action, which disturbs the metabolism of carbohydrates, fats, and proteins. Type 1 Diabetes (T1D) is an autoimmune disease where T cells mediate inflammation and specifically damage pancreatic β cells.