N-Isovaleroylglycine Analysis Service

N-Isovaleroylglycine is a significant metabolite produced during the metabolism of branched-chain amino acids, particularly as an intermediate in leucine metabolism. Changes in N-isovaleroylglycine levels are closely linked to various metabolic disorders, including branched-chain amino acid metabolism disorders like isovaleric acidemia. Accurate analysis of N-isovaleroylglycine content and metabolism is essential for understanding its mechanisms in the body and its potential applications in disease diagnosis.

 



Figure 1. Molecular Structure of N-Isovaleroylglycine

 

MtoZ Biolabs offers N-Isovaleroylglycine Analysis Service using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of N-isovaleroylglycine in complex biological matrices.

 

Analysis Workflow

 

 

 

Service Advantages

 



 

Sample Submission Requirements

Sample Types

Serum, plasma, urine, tissue homogenates, and other biological samples.

 

Sample Volume

At least 200 μL of liquid sample or 200 mg of tissue sample.

 

Sample Preservation

Store and transport samples at -80°C.

 

Applications

Metabolic Disease Research: Analyze changes in N-isovaleroylglycine levels to study its role in branched-chain amino acid metabolism disorders, such as isovaleric acidemia.

 

Disease Diagnosis: Evaluate changes in N-isovaleroylglycine levels in blood and urine as biomarkers for metabolic disorders, aiding clinical diagnosis.

 

Drug Metabolism Research: Investigate the role of N-isovaleroylglycine in drug metabolism and assess the impact of drugs on branched-chain amino acid metabolism.

 

Basic Metabolism Research: Analyze the metabolic pathways of N-isovaleroylglycine under normal and pathological conditions to explore its biological functions in the body.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on N-Isovaleroylglycine

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into N-isovaleroylglycine's extensive physiological roles through top-tier analytical techniques and expert services.