N4,2'-O-dimethylcytidine Analysis Service

N4,2'-O-dimethylcytidine (m4Cm) is a modified nucleoside present in tRNA, and is derived from cytidine, a nucleoside and a constituent of RNA. Structurally, m4Cm is a pyrimidine nucleoside, composed of a pyrimidine base (cytosine) and a ribose sugar, with two methyl groups attached at positions 4 and 2'. In cell metabolism, m4Cm is involved in the post-transcriptional modification of tRNA, which is crucial for proper protein synthesis and cellular function. Many recent studies have highlighted the potential of modified nucleosides, such as m4Cm, as biomarkers for various diseases, including cancer and infectious diseases.

 



 

Figure 1. The Structure of N4,2'-O-dimethylcytidine

 

The analysis of m4Cm and other modified nucleosides can provide valuable insights into cellular metabolism and RNA function. Additionally, the detection and quantification of m4Cm in biological samples could be used to diagnose and monitor disease progression or response to treatment. As such, the analysis of m4Cm is of high scientific and clinical relevance.

 

At MtoZ Biolabs, we offer a comprehensive N4,2'-O-dimethylcytidine Analysis Service, employing advanced mass spectrometry techniques and rigorous data analysis methods to accurately detect and quantify m4Cm in your samples.

 

Analysis Workflow

 



 

Service Advantages

• State-of-the-art LC-MS/MS platforms allow us to accurately and reliably quantify N4,2'-O-dimethylcytidine in various types of samples.
• A team of experts in mass spectrometry and metabolomics provides professional data analysis and interpretation.
• One-stop service from sample preparation, LC-MS/MS analysis to data analysis, saving your time and efforts.
• Competitive price with high-quality service.

 

Applications

Our N4,2'-O-dimethylcytidine Analysis Service can be applied in various fields, such as: 

• Study of RNA modifications in cellular processes.
• Exploration of epitranscriptomics and gene regulation.
• Investigating RNA stability and degradation pathways.
• Analysis of nucleotide modifications in cancer research.
• Evaluation of RNA modifications in metabolic diseases.
• Understanding the role in immune response modulation.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on N4,2'-O-dimethylcytidine

4. Raw Data

5. Custom Analysis Report

 

Choose MtoZ Biolabs for your N4,2'-O-dimethylcytidine analysis needs. Our dedicated team is ready to provide you with accurate and reliable results. Contact us today to learn more about our services.