O-Glycosylation Site Analysis Service | Biological Products

Unlike N-glycans, O-glycans are smaller, highly diversified branched carbohydrate molecules that can attach to various protein structures. This characteristic leads to the complexity of the analysis, making it difficult to analyze O-glycosylation sites. The variability of glycosylation sites is a key indicator of cellular activity, as evidenced by the correlation between the reduction in serum protein glycosylation sites and the severity of congenital disorders of glycosylation. Therefore, accurately determining site occupancy is crucial for understanding the impact of protein glycosylation on human health. In biopharmaceutical development, the analysis of O-glycosylation is essential for characterizing glycosylation patterns of drug molecules, ensuring product consistency, and maintaining quality.

 

MtoZ Biolabs offers O-Glycosylation Site Analysis Service, providing comprehensive insights into the O-glycosylation status of proteins, thereby supporting the development and manufacturing of biopharmaceuticals.

 

Analysis Workflow

The general steps in O-glycosylation site analysis include the proteolytic digestion of glycoproteins, enrichment and separation of glycopeptides via hydrophilic interaction liquid chromatography (HILC). Enriched O-glycopeptides can be analyzed using reversed-phase liquid chromatography-ion trap mass spectrometry (RPLC-ITMS).

 



Huang, L, J. et al. J. Chromatogr. A. 2014.

Figure 1. General Analysis Procedure for O-glycosylation Site Attachment

 

Applications

1. Biopharmaceutical Development and Optimization: Enhancing drug efficacy, stability, and safety by analyzing O-glycosylation patterns.
2. Quality Control and Regulatory Compliance: Ensuring product quality and regulatory compliance through consistent monitoring of O-glycosylation modifications.
3. Biomarker Discovery: Identifying O-glycosylation modifications associated with specific diseases for biomarker development and validation.
4. Protein Function Research: Examining the impact of O-glycosylation on protein activity, interactions, and stability.

 

Deliverables

1. Experimental Procedures

2. Mass Spectrometry Parameters

3. Mass Spectrometry Images

4. Raw Data

5. Detailed Information on Identified Glycosylation Sites

 

MtoZ Biolabs has much analytical service experience in glycomics and can customize glycomics analysis services according to your specific needs. Free project evaluation!