O-Link Proteomics
O-Link Proteomics is a cutting-edge research methodology leveraging proximity extension assay (PEA) technology to conduct high-throughput and precise measurements of protein dynamics in biological processes. Developed by Olink, this PEA platform allows for the simultaneous quantification of multiple protein expressions. A hallmark of O-Link Proteomics is its high sensitivity and specificity. Traditional proteomics approaches often require large sample volumes and struggle with low detection limits and specificity. Olink's approach ameliorates these issues through the strategic application of dual antibody binding, allowing researchers to glean extensive protein insights from minimal sample amounts—a crucial advantage in contexts where samples are limited or challenging to obtain, such as cerebrospinal fluid and tissue sections. The high-throughput nature of O-Link Proteomics also facilitates the concurrent measurement of hundreds of proteins, enabling comprehensive, multi-level analyses that advance systems biology. Its applications are extensive in clinical research, providing robust tools for elucidating disease mechanisms and identifying biomarkers. By comparing protein expression profiles across patient cohorts, researchers can pinpoint disease-specific protein markers, supporting personalized medicine. Furthermore, in drug development, O-Link Proteomics aids in identifying drug targets and evaluating therapeutic efficacy, thereby streamlining the development pipeline and reducing costs.
Technical Principle
O-Link Proteomics utilizes uniquely designed antibody probes, each tagged with a distinct DNA sequence. Upon specific binding of these antibodies to adjacent epitopes on a target protein, their DNA tags are brought into proximity, facilitating the formation of double-stranded DNA via a ligation reaction. The resultant DNA is quantified through quantitative PCR or next-generation sequencing, with yields directly proportional to the target protein concentration, ensuring high sensitivity and specificity.
Technical Process
1. Sample Preparation
The accuracy of O-Link Proteomics is contingent on sample quality. Thus, selecting appropriate samples and conducting necessary pretreatments, such as impurity removal and protein concentration, are foundational steps.
2. Double Antibody Binding Technology
Central to O-Link Proteomics is the double antibody binding strategy, where each target protein is matched with specific antibody pairs. These antibodies, upon binding to the target, initiate probe extension via proximity effects, allowing for precise quantitative analysis.
3. High-Throughput Detection
The PEA technology empowers researchers to analyze up to 92 proteins concurrently within a sample, vastly enhancing the scope of large-scale and complex disease studies.
4. Data Analysis
Proteomics data complexity necessitates specialized software and algorithms for analysis. Data normalization and background correction are typically required to ensure the accuracy and reproducibility of results.
Features of O-Link Proteomics Technology
1. High Sensitivity
O-Link Proteomics excels in detecting low-abundance proteins, key in understanding proteins pivotal in disease pathogenesis despite their low concentration.
2. Multi-Target Detection
This method detects multiple proteins simultaneously in a single reaction, providing comprehensive protein expression profiles that enhance research efficiency and biological insight.
3. Low Sample Requirement
Requiring only small sample volumes, such as minimal blood or cell lysate, makes O-Link Proteomics suitable for studies where samples are scarce or valuable.
4. In Situ-Like Detection
Antibody-protein interactions occur in solution, closely mirroring natural protein states within organisms, offering an authentic reflection of protein expression and interactions.
O-Link Proteomics presents unique advantages over traditional mass spectrometry-based techniques, particularly in sensitivity, multi-target detection, and sample efficiency. While mass spectrometry is capable of extensive protein identification and quantification, it struggles with low-abundance proteins and demands larger samples. In contrast, O-Link Proteomics focuses on high-sensitivity detection and quantification of specific targets, ideal for detailed investigation of known markers. MtoZ Biolabs is dedicated to providing advanced O-Link Proteomics services, encompassing the entire workflow from sample handling to data interpretation, aiming to facilitate breakthroughs in research and clinical applications. We look forward to potential collaborations.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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