Peptide Purity Analysis Service

Peptide purity is defined as the ratio of the target peptide to all analytes, essentially reflecting the proportion of the target peptide to impurities. It is typically determined using HPLC analysis at 220nm, the peak absorption wavelength for peptide bonds. The purity of peptides critically influences research outcomes, with potential contaminants arising during chemical synthesis, culturing, or the extraction and purification phases. Impurities may include peptides with incomplete sequences, truncated sequences, incompletely deprotected peptides, and by-products from synthesis or final cleavage processes.

 



Figure 1. 3D Structure of Peptides

 

Applications



Figure 2. Application Areas for Peptides of Different Purities

 

Services at MtoZ Biolabs

1. RP-HPLC

Chromatograms from reverse phase HPLC are utilized to assess peptide purity by quantifying the peak area of the fully purified peptide relative to the total detected peak areas, facilitating the determination of by-product quantities and proportions.

 

2. Mass Spectrometry

Chromatograms from RP-HPLC are utilized to assess peptide purity by quantifying the peak area of the fully purified peptide relative to the total detected peak areas, facilitating the determination of by-product quantities and proportions.

 

3. Amino Acid Analysis (AAA)

Determines the amino acid composition in peptide or protein products, ensuring experimental accuracy and consistency.

 

MtoZ Biolabs delivers peptide purity analysis services leveraging RP-HPLC and mass spectrometry, dedicated to high-quality results. Each project requires a unique approach, so please engage our technical team to discuss specific needs.

• • HPLC Peptide Purity Analysis Service

  High-performance Liquid Chromatography (HPLC) is a modern chromatographic separation technique extensively utilized in biological, chemical, and medical research. For peptide purity analysis, HPLC identifies the composition and content of peptides in samples, assessing the purity and impurities. Peptides are dissolved in a mobile phase and separated using a chromatographic column containing a stationary phase.