Peptide Sequencing Service for Protein Analysis
In the domain of proteomics, peptide sequencing services are instrumental in enabling researchers to decode complex protein networks and their functions. Peptide sequencing involves utilizing advanced technologies to elucidate the sequence of proteins or peptides, thereby providing researchers with detailed information regarding amino acid sequences. Sequencing and analyzing peptides produced during manufacturing processes ensure product purity and consistency, thereby maintaining the quality of biotechnological products. Accurate protein sequencing allows for a deeper investigation into the relationship between protein structure and function, offering substantial data support for life science research. In drug development, peptide sequencing services aid scientists in analyzing and optimizing protein target structures, thus informing new drug development efforts. In studies of disease mechanisms, researchers can uncover protein alterations under pathological conditions through peptide sequencing, providing valuable insights for disease diagnosis and treatment. Peptide sequencing service is a professional bioanalytical service, detailed as follows:
Service Offerings
1. Verification of Known Sequences
Sequencing technologies are employed to verify the expression of known peptide sequences in actual samples, checking for errors, mutations, or modifications.
2. Determination of Unknown Sequences
For peptides with undetermined sequences, various sequencing technologies such as de novo sequencing via mass spectrometry are applied to deduce their amino acid composition and sequence.
3. Analysis of Post-Translational Modifications
Beyond amino acid sequencing, this service can detect post-translational modifications, including phosphorylation, glycosylation, and acetylation, identifying modification sites and types.
Technical Approaches
1. Edman Degradation
A traditional method of peptide sequencing, Edman degradation involves sequentially removing amino acids from the N-terminal of a peptide and identifying each to determine the sequence. This method is relatively slow and typically accurate for up to 20-30 amino acids.
2. Mass Spectrometry
Tandem mass spectrometry (MS/MS) is the predominant method for contemporary peptide sequencing. Peptides or proteins are fragmented, and their fragments are analyzed using mass spectrometry. In a mass spectrometer, peptide ions are ionized, separated, and detected based on their mass-to-charge ratio (m/z). By assessing the m/z of peptide fragments and their mass differences, the amino acid sequence can be inferred.
3. Liquid Chromatography-Mass Spectrometry (LC-MS/MS)
This method combines high-performance liquid chromatography with mass spectrometry to enable rapid, high-sensitivity, high-throughput sequencing of peptides and proteins.
Through the analysis of peptide sequence variations in biological samples, disease-specific peptide markers can be identified, aiding early diagnosis, monitoring, and prognostic evaluations. MtoZ Biolabs offers professional peptide sequencing services, leveraging advanced detection platforms and extensive expertise to deliver high-quality sequencing results and comprehensive technical support. We are dedicated to developing tailored solutions for each client, facilitating advancement in research and production. We invite researchers from various fields to collaborate with us in furthering scientific progress.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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