Peptidomic Service

Peptidome is a discipline dedicated to studying endogenous peptides within biological systems or protein degradation products under specific conditions. Peptides typically refer to sequences smaller than 10 kDa. Current research focuses on endogenous peptides, low-molecular-weight proteins, bioactive peptides, disease-specific peptides, and protein degradation products. Peptidomic service investigates these peptide fragments to uncover the biological significance of cytokines, antimicrobial/antiviral peptides, flavor peptides, and disease biomarkers, playing a crucial role in medicine, food science, and other fields. MtoZ Biolabs provides peptidomics sequencing services, integrating bioinformatics, proteomics, and mass spectrometry-based sequencing analysis to comprehensively characterize and quantify peptides.

 



Figure 1. Research Objects of Peptidomics.

 

Analysis Workflow

The fundamental approach for peptidomics and proteomics sequencing services is essentially the same. The experimental workflow for peptidomic service consists of the following steps: First, the sample undergoes preprocessing to extract peptides. Common extraction methods include size exclusion chromatography, ultrafiltration, and organic precipitation. Once the peptides are obtained, the sample is subjected to reduction and alkylation treatment to break disulfide bonds, facilitating subsequent peptide detection. Next, the purified peptides are introduced into a liquid chromatography-mass spectrometry (LC-MS) system for mass spectrometric analysis, generating spectra. These spectra are then searched against a spectral database using specialized software to obtain qualitative and quantitative information on the peptides. Finally, bioinformatics methods are applied to analyze various properties of the peptide samples, providing as much relevant information as possible to clients.

 



Figure 2. Experimental Flow of Peptidomics.

 

It is worth noting that in the entire peptidomic service experimental workflow, enzymatic digestion is generally not performed. Instead, the database search parameters are typically set to "unspecific" to ensure more accurate spectral library predictions. The peptidomic service provided by MtoZ Biolabs consider the research needs of many customers for small peptides. During the mass spectrometry acquisition process, we will relax the collection range to 100-1500m/z to ensure effective collection of small peptides. In the final deliverables, MtoZ Biolabs provides not only qualitative and quantitative peptide results but also multiple physicochemical properties of peptides, including peptide length, amino acid composition, isoelectric point, and hydrophobicity. Additionally, based on client requirements, we offer both de novo sequencing and database search strategies.

 

Sample Submission Suggestions

1. Sample Types

MtoZ Biolabs accepts a wide range of sample types, including tissues (animal and plant tissues), microorganisms, body fluids, cell pellets, protein solutions, and culture supernatants.

 

2. Sufficient Sample Preparation

Note: Samples should be shipped on dry ice. The standard sample submission follows routine experimental procedures. If the sample quantity is insufficient or if you have other sample types, please contact our technical support team for a free consultation.

 

Deliverables

MtoZ Biolabs not only provides accurate peptidomics sequencing services but also offers comprehensive post-service support. For peptidomics sequencing services, we deliver the following:

 

1. Comprehensive Experimental Details

 

2. Materials, Instruments, and Methods

 

3. Total Ion Chromatogram & Quality Control Assessment

 

4. Data Analysis, Preprocessing, and Estimation 

 

5. Bioinformatics Analysis

 

6. Raw Data Files

 

In addition to peptidomic service, MtoZ Biolabs also offers additional paid analytical services, including various types of bioactivity predictions (such as peptide sequence prediction, glycosidase inhibitory peptide prediction, antioxidant prediction, anti-inflammatory peptide prediction, toxic peptide prediction, and bitter peptide prediction) as well as molecular docking services. Further customized analyses can be evaluated based on specific requirements.

 

The above provides a brief introduction to the peptidomic service workflow. In addition, we also offer services in proteomics, metabolomics, genomics, and drug characterization. MtoZ Biolabs is a mass spectrometry-based multi-omics service provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. We are equipped with mainstream high-resolution mass spectrometry platforms, including 480, Astral, and TimsTOF, ensuring highly accurate data for our clients. Our ultimate goal is to provide fast, high-throughput, and cost-effective analyses with outstanding data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!