Perform Protein Mass Spectrometry After IP

Immunoprecipitation (IP) is a method of purifying proteins. The principle is to incubate the antibody of the target protein with the sample extract, allowing the antibody and protein to bind in the solution. Then the antibody/antigen complex is extracted from the sample with agarose gel coupled with bacterial protein A/G.

 

IP mainly captures target proteins and their interacting proteins selectively. If you want to quantify these proteins, you need to cooperate with other technologies. In recent years, with the development of mass spectrometry (MS) technology, it has become a common trend to combine MS with other technologies for protein research.

 

After IP, mass spectrometry analysis can further identify and quantify the enriched proteins. The process is to first digest the protein complex obtained by IP to obtain a series of protein fragments (peptides). Then, the peptide mixture after protein enzymatic hydrolysis is ionized, separated, and detected by a mass spectrometer. By analyzing the mass-to-charge ratio (m/z) and mass spectrometry data of peptides, qualitative and quantitative analysis of proteins can be achieved.

 

MtoZ Biolabs is a professional biological mass spectrometry service provider, which can provide one-stop detection of interactive proteins, including protein interaction experiments, IP, CO-IP, GST pull-down to one-stop completion of protein sample mass spectrometry analysis. You only need to send your requirements and samples to us, and we will be responsible for all subsequent matters of the project, including sample pretreatment, protein interaction experiments, mass spectrometry analysis, and mass spectrometry raw data analysis. We welcome your consultation.