Phospho Proteomics Analysis Service

Phospho proteomics utilizes high-resolution mass spectrometry and specialized enrichment strategies to precisely identify phosphorylated proteins and their modification sites, enabling researchers to uncover complex biological regulatory mechanisms. Phosphorylation is a dynamic and reversible post-translational modification that plays a pivotal role in key biological processes such as signal transduction, cell cycle regulation, and metabolic control, and is closely associated with the development of various diseases. Phospho proteomics is a critical technology for studying protein phosphorylation modifications, offering comprehensive insights into phosphorylation sites and regulatory networks within cells. This analysis holds significant value in signal pathway research, biomarker discovery, and drug target development, providing robust technical support for basic research and precision medicine.

 

Service at MtoZ Biolabs

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. MtoZ Biolabs is honored to offer phospho proteomics analysis service utilizing a robust and professional platform featuring the Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos mass spectrometry systems, coupled with a Nano-LC system. Our service include global phosphoproteome analysis, quantitative phosphoproteomics, top-down/middle-down/bottom-up phospho proteomics, targeted phosphorylation site validation, etc. If you are interested in our service or have other analysis needs, please contact us freely.

 

Analysis Workflow

 



Riley, NM. et al. Anal Chem. 2016.

 

Service Advantages

1. Efficient Phosphopeptide Enrichment Techniques  

MtoZ Biolabs employs optimized phosphopeptide enrichment strategies (e.g., TiO₂, IMAC) and advanced sample preparation methods to significantly enhance the detection sensitivity of low-abundance phosphopeptides, ensuring comprehensive capture of critical modification information.

 

2. State-of-the-Art Mass Spectrometry Platforms  

Leveraging high-resolution mass spectrometers such as the Thermo Fisher Orbitrap Fusion Lumos and Q Exactive HF, MtoZ Biolabs delivers precise identification of phosphorylated proteins and their modification sites. Our services support top-down, middle-down, and bottom-up analyses, enabling reliable insights into the dynamic relationships between phosphorylation sites.

 

3. Expert Data Analysis and Functional Validation  

MtoZ Biolabs is equipped with an experienced bioinformatics team, utilizing advanced algorithms to analyze large-scale data. We also offer customized services such as kinase substrate screening and specific site validation, empowering researchers to fully interpret the biological significance of phosphorylation modifications.

 

Applications

Phospho proteomics can be used in:

1. Revealing Dynamic Signaling Networks and Bioenergetics Pathways

 



Tan, H. et al. Immunity. 2017.

 

2. Identifying Disease Marker

 



Hadisurya, M. et al. Commun Med (Lond). 2023.

 

3. Assisting in Drug Target Development

 



Vallés-Martí, A. et al. Cell Rep. 2023.

 

4. In Precision Medicine

 



Giudice, G. et al. Brief Bioinform. 2019.

 

FAQ

1. How to resolve the relationship between distantly located key phosphorylation sites?  

Middle-down and top-down proteomics are the core methods to address this issue. These techniques avoid fully digesting proteins into small peptides, enabling direct observation of multiple key sites along a long sequence. For example, in the case of Src family kinases, top-down approaches can reveal whether the C-terminal inhibitory site and the activation loop phosphorylation coexist, providing insights into their potential coordinated regulation of protein activity. Further functional experiments, such as site-directed mutagenesis or protein activity assays, can validate the biological impact of these phosphorylation patterns. On the technical front, the use of high-resolution instruments such as FTICR-MS, combined with optimized software algorithms, significantly enhances the interpretability and precision of the data.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Phospho Proteomics Analysis

5. Mass Spectrometry Image

6. Raw Data