Plant Single-Cell Proteomics Analysis
Single-cell proteomics in plants is a cutting-edge research technology that provides a comprehensive and precise analysis of protein expression in individual plant cells. This technique offers critical insights into protein dynamics during various stages of plant development, environmental stress response, and pathogen infection. Compared to traditional bulk proteomics, single-cell proteomics overcomes the challenge of cellular heterogeneity, enabling researchers to understand biological mechanisms at the cellular level. This method is highly valuable in research areas such as plant developmental biology, cellular signaling, environmental adaptation, and plant pathology.
Services at MtoZ Biolabs
As a leader in mass spectrometry analysis, MtoZ Biolabs offers high-precision Single-Cell Plant Proteomics analysis services, leveraging our advanced mass spectrometry platform and extensive technical expertise. Our services address the following critical challenges:
✅ Decoding plant cellular heterogeneity and revealing differences among cell types during development and growth.
✅ Quantitatively analyzing dynamic protein changes in single cells under environmental stress to uncover mechanisms of stress tolerance.
✅ Deciphering hormonal signaling pathways at the cellular level, advancing the understanding of plant biology.
With our services, clients can access precise, in-depth proteomics data that support the successful advancement of their research projects.
Analysis Workflow
Service Advantages
✅ Advanced Technology, Tailored for Plant Systems
We understand the unique challenges of plant single-cell research, particularly in protein extraction and sample preparation. Our optimized single-cell sample preparation techniques, combined with a high-sensitivity LC-MS/MS platform, provide in-depth analysis specifically designed for plant systems. Whether it is detecting low-abundance proteins in complex samples or uncovering intricate molecular regulatory networks, our state-of-the-art mass spectrometers, such as the Orbitrap Fusion Lumos, ensure high sensitivity and comprehensive coverage at the single-cell level.
✅ Customized Solutions to Meet Your Research Needs
Every research project is unique, and we offer flexible, customized services to meet your specific requirements. From single-cell isolation to proteomics analysis, our team works closely with you to tailor the most suitable experimental plan, ensuring the reliability and reproducibility of results. We offer comprehensive, end-to-end services covering everything from sample preparation to data interpretation, helping you turn ideas into tangible scientific outcomes
✅ In-Depth and Professional Data Analysis
Our expert data analysis team provides diverse downstream analyses, including differential protein analysis, functional enrichment, and protein interaction network construction. We deeply explore each single-cell dataset to help you discover key biological signals, offering comprehensive bioinformatics support to take your research to the next level.
✅ Efficient Service and Data Confidentiality
We are committed to delivering efficient services to ensure that your project receives reliable data in the shortest possible time. At the same time, we strictly guarantee the security and confidentiality of client data, ensuring that your research outcomes are fully protected.
Choose us, and let us empower your plant single-cell proteomics research as we advance the frontiers of plant science together!
Sample Results
1. Single-cell Proteomics Differentiates Arabidopsis Root Cell Types
SCP is an emerging approach to resolve cellular heterogeneity within complex tissues of multi-cellular organisms. The Arabidopsis root represents a well-characterized organ with defined cell types, making it an excellent system for benchmarking SCP in plants. We examined single cells isolated from the cortex and endodermis of Arabidopsis roots using SCP and identified 3763 proteins from 756 root cells, of which 596 proteins were enriched in either the cortex or endodermis and were able to distinguish between these closely related plant cell types. This study demonstrates that SCP can resolve neighboring cell types with distinct functions, thereby facilitating the identification of biomarkers and candidate proteins to enable functional genomics.
Montes C, et al. New Phytol. 2024.
Differences in the Proteomes of Endodermal and Cortical Cells in Arabidopsis Roots
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
With our cutting-edge mass spectrometry platform and expert technical team, we are dedicated to delivering high-quality single-cell proteomics analysis services for plant research. Whether you are conducting research at the single-cell level or seeking deeper insights into plant molecular biology or cellular biology, we invite you to contact us for more information! Let's work together to advance plant science research.
How to order?
