PRM-MS Mass Spectrometry
PRM-MS (Product Ion Monitoring Mass Spectrometry) is a mass spectrometry technique extensively applied in quantitative bioanalysis, particularly in proteomics and peptide mass spectrometry. It is highly regarded for its exceptional sensitivity, accuracy, and reproducibility.
PRM-MS operates as a mode of tandem quadrupole mass spectrometry. During PRM-MS analysis, precursor ions of the target molecules are initially selected. These ions then undergo either Collision Induced Dissociation (CID) or Higher-energy Collisional Dissociation (HCD), resulting in the formation of numerous product ions. The masses and relative abundances of these product ions are recorded via a comprehensive scan. This approach provides extensive structural information, facilitating the identification and quantification processes. Consequently, PRM-MS is considered a leading technique in quantitative analysis today.
Applications
Thanks to its remarkable sensitivity, accuracy, and reproducibility, PRM-MS is widely employed in the analysis of biological samples. It finds applications in areas such as proteomics, metabolomics, drug metabolism research, clinical diagnostics, and environmental pollutant detection. For instance, in proteomics, PRM-MS is utilized for quantitative analysis of protein expression levels and studies on protein modifications. In metabolomics, the technique is employed to detect and quantify various metabolites, thereby aiding in the exploration of changes in metabolic pathways within organisms.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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