Protein 2-Hydroxyisobutyrylation Analysis

Post-translational modifications (PTMs) involve chemical changes to proteins during or after their synthesis, playing crucial roles in cellular processes such as differentiation, degradation, signal transduction, gene expression regulation, and protein-protein interactions. 2-Hydroxyisobutyrylation (2HIB), a novel PTM first identified in histones, involves the covalent attachment of a 2-hydroxyisobutyryl group to lysine residues in proteins. This modification significantly impacts gene expression, metabolic pathways, and signal transduction. Investigating the mechanisms of 2-hydroxyisobutyrylation through target protein hydroxyisobutyrylation analysis and hydroxyisobutyrylation proteomics is vital for understanding complex biological processes and developing innovative therapeutic strategies.

 

Services at MtoZ Biolabs

MtoZ Biolabs utilizes Thermo Fisher's Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometry platforms, in combination with Nano-LC, to offer a comprehensive solution for protein 2-hydroxyisobutyrylation analysis, which include target protein 2-hydroxyisobutyrylation analysis and 2-hydroxyisobutyrylation proteomics. Simply provide us with your experimental objectives and sample materials, and we will manage the entire workflow, delivering detailed identification and quantification of 2-hydroxyisobutyrylation modifications on specific proteins and a panoramic view of 2-hydroxyisobutyrylation modifications across various samples, uncovering extensive 2-hydroxyisobutyrylation patterns. If you are interested in our protein 2-hydroxyisobutyrylation analysis service, please feel free to contact us. We are dedicated to providing you with the best service.

 

Analysis Workflow

 



 

Service Advantages

1. Advance Analysis Platform: Mtoz Biolabs established an advanced protein 2-hydroxyisobutyrylation analysis analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein 2-hydroxyisobutyrylation analysis data providing clients with acomprehensive data report.

 

4. Comprehensive Analysis: Both target protein 2-hydroxyisobutyrylation analysis and 2-hydroxyisobutyrylation proteomics can be conducted in MtoZ Biolabs.

 

Sample Submission Requirements

 



1. If you are providing tissue samples, please ship them to us on dry ice.

 

2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.

 

3. Sample transportation: Please ensure that samples are shipped with sufficient dry ice and choose the fastest possible shipping way to minimize the risk of sample degradation during transit.

 

4. Before proceeding with the formal experiment, we will test the samples you provide, and the formal analysis will begin once the samples pass the quality check.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. The Detailed Information of Protein 2-Hydroxyisobutyrylation Analysis

4. Mass Spectrometry Image

5. Bioinformatics Analysis

6. Raw Data