Protein Amino Acid Analysis Service
- Dry protein samples: ≥ 100 µg, with a concentration of approximately 0.5-2 mg/mL.
- Liquid material: >100 μL at a minimum concentration of 0.5 mg/mL.
Amino acids are the fundamental building blocks of proteins, essential to all living organisms. They consist of an amino group (-NH2), a carboxyl group (-COOH), a hydrogen atom, and a distinctive side chain (R-group) attached to a central carbon atom. There are 20 standard amino acids, each differing in their R-groups, which confer unique chemical properties and biological functions. These diverse structures enable proteins to adopt complex three-dimensional shapes, facilitating their roles in catalysis, structural support, signaling, and regulation within cells. Protein Amino Acid Analysis Service is a comprehensive analytical service used to identify and quantify the amino acid composition of proteins and peptides. This process involves hydrolyzing the protein into its constituent amino acids, which are then separated, detected, and quantified using advanced chromatographic and spectrometric methods. Amino acid analysis provides critical information about the protein’s primary structure, including its amino acid sequence, composition, and any post-translational modifications. This detailed analysis is indispensable for characterizing proteins, verifying their purity, and ensuring their functionality in various applications.
Figure 1. The Structure of Amino Acid
Figure 2. Formation of Proteins: From Amino Acids to Peptides to Proteins
Services at MtoZ Biolabs
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, providing advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption.
At MtoZ Biolabs, with over a decade of amino acid analysis expertise, we provide advanced protein amino acid analysis services using technologies like iTRAQ, SRM/MRM, ion-exchange LC, reverse-phase HPLC, gas chromatography (GC), capillary electrophoresis (CE), strong cation exchange (SCX), hydrophilic interaction chromatography (HILIC), ultraviolet (UV) and fluorescence (FL) spectrophotometry, ESI-MS/MS, MALDI TOF MS, and TOF/TOF MS/MS. Our state-of-the-art automatic amino acid analyzers and high-throughput LC-MS/MS systems ensure accurate identification and quantification of 20 proteinogenic amino acids as well as other amino acids in biological compounds, biological fluids, drugs, tissues, food, microbiology samples, environmental samples, and more. Trust MtoZ Biolabs for precise and reliable amino acid analysis tailored to your research and clinical needs.
Lam, A. K. et al. Mol Ther Methods Clin Dev. 2022.
Figure 3. Workflow for Amino Acid Analysis
Service Advantages
1. Advance Analysis Platform: MtoZ Biolabs established an advanced protein amino acid analysis service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein amino acid analysis data providing clients with a comprehensive data report.
4. Fast Turnaround: Efficient workflows and dedicated resources enable us to deliver timely results without compromising quality.
Applications
1. Protein Characterization
Amino acid analysis helps determine the exact amino acid composition and sequence of proteins, which is essential for understanding their structure and function.
2. Quality Control
In the biopharmaceutical industry, amino acid analysis ensures the purity and consistency of therapeutic proteins, vaccines, and biologics, meeting stringent regulatory standards.
3. Research and Development
Researchers utilize amino acid analysis to study protein synthesis, degradation, and modifications, advancing our knowledge in fields like enzymology, molecular biology, and biotechnology.
4. Validation of Recombinant Proteins
Amino acid analysis verifies that recombinant proteins are correctly translated, ensuring their suitability for downstream applications.
Sample Submission Suggestions
Note: Feel free to contact us if you have any additional sample requirements.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
FAQ
Q1: Can protein amino acid analysis service be used to determine protein folding or conformation?
Protein amino acid analysis service provides quantitative information on the individual amino acid composition of a protein sample but does not directly offer insights into protein folding or conformation. Techniques such as circular dichroism or X-ray crystallography are more suitable for analyzing protein folding and structural conformation.
Q2: How does amino acid analysis compare to other protein analysis techniques, such as SDS-PAGE or Western blotting?
Amino acid analysis provides quantitative data on the individual amino acid content within a protein sample, whereas SDS-PAGE and Western blotting offer information regarding the molecular size, properties, and quantity of proteins. These techniques are complementary and can be used together to achieve a more comprehensive understanding of protein composition and quality.
How to order?
