Protein Butyrylation Analysis

Post-translational modifications (PTMs) refer to chemical changes made to proteins during or after their synthesis, playing critical roles in various cellular processes, including cell differentiation, protein degradation, signal transduction, gene expression regulation, and protein-protein interactions. Lysine butyrylation (Kbu), an emerging PTM, occurs through the covalent attachment of a butyryl group to lysine residues in proteins. This modification is crucial for regulating gene expression, metabolism, epigenetics, and cellular differentiation. Investigating lysine butyrylation (including target protein butyrylation analysis and butyrylation proteomics) can enhance our understanding of its biological significance in health and disease, potentially offering novel therapeutic strategies for metabolic disorders and cancer.

 

Services at MtoZ Biolabs

MtoZ Biolabs utilizes Thermo Fisher's Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometry platforms, in combination with Nano-LC, to offer a comprehensive solution for protein butyrylation analysis, which include target protein butyrylation analysis and butyrylation proteomics. Simply provide us with your experimental objectives and sample materials, and we will manage the entire workflow, delivering detailed identification and quantification of butyrylation modifications on specific proteins and a panoramic view of butyrylation modifications across various samples, uncovering extensive butyrylation patterns. If you are interested in our histone lysine methacryloylation analysis service, please feel free to contact us. We are dedicated to providing you with the best service.

 

Analysis Workflow

1. Sample Preparation: Proteins are extracted from cells or tissues and lysed using a buffer containing protease and deacetylase inhibitors to preserve the butyrylation modifications.

 

2. Protein Digestion: Trypsin is used to digest the proteins into peptides for subsequent mass spectrometry analysis.

 

3. Butyrylated Peptide Enrichment: Specific anti-butyrylation antibodies are used to enrich butyrylated peptides, minimizing interference from non-modified peptides.

 

4. Mass Spectrometry Analysis (LC-MS/MS): Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is employed to detect and quantify the enriched butyrylated peptides and identify their modification sites.

 

5. Data Analysis: The mass spectrometry data are analyzed using appropriate software to determine the precise locations and abundance of butyrylation modifications.

 

Service Advantages

1. Advance Analysis Platform: Mtoz Biolabs established an advanced histone asymmetric dimethylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all histone asymmetric dimethylation analysis data providing clients with acomprehensive data report.

 

4. Comprehensive Analysis: Both target protein butyrylation analysis and butyrylation proteomics can be conducted in MtoZ Biolabs.

 

Sample Submission Requirements

 



1. If you are providing tissue samples, please ship them to us on dry ice.

 

2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.

 

3. Sample transportation: Please ensure that samples are shipped with sufficient dry ice and choose the fastest possible shipping way to minimize the risk of sample degradation during transit.

 

4. Before proceeding with the formal experiment, we will test the samples you provide, and the formal analysis will begin once the samples pass the quality check.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. The Detailed Information of Protein Butyrylation Analysis

4. Mass Spectrometry Image

5. Bioinformatics Analysis

6. Raw Data