Protein Carboxyethylation Analysis

Protein carboxyethylation is a significant post-translational modification (PTM) where a carboxyethyl group (-CH₂-COOH) is covalently added to lysine or cysteine residues, altering protein structure and function. Recent research has demonstrated that this modification generates neoantigens, inducing HLA-restricted autoimmune responses, particularly playing a crucial role in autoimmune diseases such as ankylosing spondylitis (AS).

 



Zhai, Y. et al. .Science. 2023.

Figure 1. Cysteine Carboxyethylation Provokes HLA-Restricted Autoimmune Responses in Ankylosing Spondylitis

 

MtoZ Biolabs, utilizing advanced mass spectrometry platforms and specialized proteomic techniques, provides highly sensitive and specific protein carboxyethylation detection and quantification services, facilitating the detailed investigation of this modification in both physiological and pathological contexts.

 

Analysis Workflow

 



 

Services at MtoZ Biolabs

MtoZ Biolabs provides Protein Carboxyethylation Analysis services both at the level of specific target proteins and across the entire proteome. Our Target Protein Carboxyethylation Analysis uses immune enrichment and high-resolution mass spectrometry to precisely identify modification sites on selected proteins. Meanwhile, our Carboxyethylation Proteomics offers comprehensive, high-throughput mass spectrometry analysis to examine carboxyethylation patterns across the proteome, enabling researchers to thoroughly investigate protein modification mechanisms and drive scientific discoveries.

 

Service Advantages

1. Advance Analysis Platform

MtoZ Biolabs established an advanced protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. Wide Application

Our analysis is adaptable to numerous biological sample types, supporting the systematic study of protein carboxyethylation across diverse physiological and pathological conditions.

 

3. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate PTM proteomics analysis data providing clients with a comprehensive data report.

 

4. Customized Solutions

We offer tailored services, ranging from the identification of modification sites to comprehensive functional annotation, meeting the specific needs of various research projects.

 

5. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

Applications

Protein carboxyethylation has significant research potential in autoimmune and other pathological processes. MtoZ Biolabs’ carboxyethylation analysis services support the following areas:

• Autoimmune Disease Research: Neoantigens generated by carboxyethylation are closely linked to HLA-restricted autoimmune responses, making this service ideal for studying molecular mechanisms in diseases like ankylosing spondylitis and rheumatoid arthritis.
• Metabolic Disorder Research: By detecting changes in carboxyethylation induced by metabolic byproducts, our services contribute to the investigation of metabolic diseases such as diabetes and atherosclerosis.
• Oxidative Stress Studies: Carboxyethylation is involved in cellular responses to oxidative damage, and its quantification aids in exploring antioxidant defense mechanisms.
• Drug Development: Monitoring carboxyethylation during drug testing allows for precise assessment of therapeutic efficacy and target engagement, supporting new drug development efforts.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!