Protein Identification by LC-MS/MS Service
- Low-complexity samples: Gel spots or bands, approximately 1-2 cm in length.
- Medium-complexity samples: Protein mixtures, including column-purified samples.
- Moderate-to-high complexity samples: Protein mixtures such as IP-derived samples, secreted proteomes, or subcellular organelles (e.g., mitochondria, endoplasmic reticulum, lysosomes).
- High-complexity samples: Whole cell lysates, tissues.
- Disease biomarker discovery
- Identification of drug targets
- Protein function analysis
- Multi-omics and metabolomics studies
- Investigation of cellular signaling pathways
MtoZ Biolabs offers Protein Identification by LC-MS/MS Service, enabling precise identification and analysis of proteins from complex biological samples. Protein identification is fundamental to life sciences research, with applications spanning protein function analysis, cellular metabolism, signaling pathway exploration, and biomarker discovery. Accurate protein identification is critical for advancing disease research, drug development, and metabolomics.
Mass spectrometry, particularly liquid chromatography-tandem mass spectrometry (LC-MS/MS), offers distinct advantages in protein identification. This technique enables high-sensitivity, high-resolution protein identification, and the analysis of complex samples, providing comprehensive qualitative and quantitative data. Unlike traditional methods, LC-MS/MS does not rely on antibodies, offers broader applicability, and allows for the simultaneous analysis of multiple proteins, making it particularly useful for identifying low-abundance proteins.
MtoZ Biolabs employs Thermo Fisher's Q Exactive HF and Orbitrap Fusion Lumos mass spectrometry systems in combination with Nano-LC technology, providing ultra-high precision and sensitivity in protein identification. This state-of-the-art platform allows us to efficiently separate and accurately identify proteins, facilitating deeper insights into biological mechanisms and accelerating scientific discovery.
Technical Principles
LC-MS/MS is a high-sensitivity technology combining liquid chromatography and mass spectrometry. In protein identification, liquid chromatography separates peptide fragments from complex samples, ensuring efficient entry into the mass spectrometer for individual analysis. The mass spectrometer measures the mass-to-charge ratio (m/z) of these peptides to provide initial sequence data.
Tandem mass spectrometry (MS/MS) further breaks the peptides into ion fragments through collision-induced dissociation (CID). The mass-to-charge ratios of these fragments offer structural insights into the peptide. By comparing these data with protein databases, LC-MS/MS allows accurate identification of the peptide sequence, enabling protein identification.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform: MtoZ Biolabs established an advanced protein identification by LC-MS/MS platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. Comprehensive Database Support: We utilize globally recognized protein databases, such as UniProt, to ensure the accuracy and reliability of protein identification results.
3. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.
4. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
Categories of LC-MS/MS-Compatible Samples:
For more specific sample submission requirements, please consult our technical team.
Applications
Case Study
1. Protein Identification in Bladder Cancer Patients and Healthy Controls via LC/MS-MS for Key Glycoprotein Biomarker Discovery
Yang, N. et al. Clin. Cancer Res. 2011.
2. Identification and Characterization of Hypothesized Proteins in Human Tumor Cell Lines by LC-MS/MS technology
Ijaq, J. et al. J Proteomics. 2020.
FAQ
Q1: How does LC-MS/MS technology achieve high accuracy in protein identification, particularly for complex samples?
MtoZ Biolabs utilizes Nano-LC-MS/MS technology, which is optimized for accurate protein identification in complex samples. Nano-LC provides superior resolution compared to traditional LC methods, enabling precise separation of peptides from trace samples, thus significantly improving detection sensitivity and specificity. For complex samples such as whole-cell lysates or tissue extracts, Nano-LC minimizes sample loss through nanoscale flow rates and efficient column separation, ensuring peptides are well-concentrated prior to MS/MS analysis. Coupled with tandem mass spectrometry’s deep fragmentation capabilities and comprehensive database matching, Nano-LC-MS/MS enables precise identification of low-abundance proteins amidst complex matrices, establishing a solid data foundation for subsequent analyses.
Deliverables
1. Comprehensive Experimental details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs offers precise Protein Identification by LC-MS/MS Service, supporting diverse life sciences research needs with reliable results. For more information or to discuss your specific research needs, feel free to contact us for a free consultation. We're here to support your scientific endeavors.
How to order?
