Protein Isoelectric Point Analysis in Adipose Tissue via CIEF

Adipose tissue, as the main energy storage organ in the human body, carries out multiple physiological functions, including energy storage, hormone secretion, and regulation of whole-body metabolic processes. In recent years, with the increasing prevalence of obesity and related metabolic diseases, proteomic analysis of adipose tissue has become a research hotspot. These studies not only help to understand the role of adipose tissue in health and disease but also reveal molecular mechanisms related to obesity, diabetes, cardiovascular diseases, etc.

 

The isoelectric point of the protein is closely related to its amino acid sequence and three-dimensional structure, and the isoelectric point of a specific protein is fixed. Therefore, the structure and functional differences of them can be studied by comparing the isoelectric points of different proteins. MtoZ Biolabs uses advanced Capillary Isoelectric Focusing (cIEF) technology to provide a precise and efficient analytical method for studying proteins in adipose tissue.

 

Technology Introduction

cIEF technology is an analytical method that focuses proteins to their isoelectric point under specific pH conditions. Proteins can be effectively separated according to the difference of their isoelectric points through this technology. 

 

1. Equipment Configuration

BECKMAN COULTER PA800+ Capillary Electrophoresis Instrument

 

2. Reagent Configuration

Phosphoric acid, glacial acetic acid, sodium hydroxide, arginine, urea, iminodiacetic acid, Pharmalyte 3-10 for IEF, CIEF Gel, etc.

 

Application

1. Metabolic Regulation

By analyzing the proteins in adipose tissue, we can better understand how adipocytes participate in the body's energy balance and metabolic regulation.

 

2. Disease Mechanism

Study the relationship between metabolic diseases such as obesity, diabetes, and the expression and functional changes of proteins in adipose tissue.

 

3. Drug Targets

Identifying specific proteins in adipose tissue can provide potential targets for the development of drugs to treat metabolic diseases.

 

Technical Advantages

1. High Resolution

cIEF technology can separate proteins in adipose tissue with high resolution, ensuring the accuracy of the results.

 

2. High Sensitivity

High sensitivity detection of protein isoelectric point, enabling effective analysis even for trace samples.

 

3. Fast Speed and Accuracy

Compared with traditional methods, cIEF technology is faster and more accurate, suitable for large-scale sample analysis.

 

MtoZ Biolabs has made significant progress in the analysis of protein isoelectric points in adipose tissue using cIEF technology. This not only promotes the research of metabolic diseases but also provides important support for new drug development and clinical treatment. We look forward to collaborating with more researchers to explore the mysteries of adipose tissue jointly.