CE-SDS Protein Isoform Analysis Service

Protein isoform analysis is a method to study the differences in structure and function of proteins. Proteins are key molecules in life activities, and their functions are closely related to their structures. However, the same amino acid sequence may form multiple different three-dimensional structures, which are called isoforms. Protein isoforms may result from various factors, such as post-translational modifications, heterologous expression, environmental factors, etc. Isoformization of monoclonal antibodies (mAbs) is one of the common pathways of protein degradation. Isoforms can not only cause changes in protein function but are also associated with various diseases, such as neurodegenerative diseases, cancer, etc. Therefore, the analysis and identification of protein isoforms are crucial, especially in drug research and development and protein drug production.

 

Capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) technique is effective in detecting and differentiating protein isoforms. Due to the differences in charge, size, and shape among protein isoforms, they migrate at different rates in an electric field, allowing the separation, detection, and identification of these isoforms by CE-SDS. The advantage of this technique lies in its ability to analyze both purified protein samples and complex biological samples, such as cell lysates or tissue extracts. The high resolution of CE-SDS enables it to distinguish isoforms with very similar structure. It provide a powerful tool for the structural and functional study of protein isoforms with high sensitivity, resolution, and speed.

 

MtoZ Biolabs has developed and validated a protein isoform CE-SDS analysis platform based on high-precision capillary electrophoresis instrumentation and protein electrophoresis principles, offering CE-SDS protein isoform analysis services. This service can be used to analyze protein products such as monoclonal monomers under normal and stressful conditions, aiding in the early identification of degradable drug candidates during drug development.

 

Service at MtoZ Biolabs

1. Experimental Procedures

2. Relevant Experimental Parameters

3. Detailed Information on Protein Isoforms

4. Electrophoresis Images

5. Raw Data