Protein Methacrylation Analysis
- Mechanistic studies of disease pathways
- Screening and validation of therapeutic targets
- Investigations into gene expression regulation and chromatin architecture
- Metabolic regulation research
- Biomarker identification and discovery
Protein methacrylation is a newly identified type of lysine acylation modification, classified under post-translational modifications (PTMs). This modification occurs when a methacryloyl group is covalently bound to lysine residues via glutaryl transferase, influencing key biological processes such as gene expression, chromatin remodeling, protein-protein interactions, and signal transduction. Methacrylation shares isomeric similarity with lysine crotonylation (Kcr), as both exhibit an identical mass shift (+68.023 Da) in mass spectrometric analyses. However, their subtle structural variations confer distinct biological functions and regulatory mechanisms, making the ability to distinguish between these modifications critical for understanding their roles in disease and cellular processes.
Figure 1. Lysine Methacrylation and Crotonylation
At MtoZ Biolabs, we provide high-sensitivity Protein Methacrylation Analysis services using cutting-edge liquid chromatography-mass spectrometry (LC-MS/MS) technologies. Our advanced methods enable precise differentiation between these isomeric modifications, allowing researchers to uncover the unique roles of methacrylation across various biological processes.
Analysis Workflow
Services at MtoZ Biolabs
MtoZ Biolabs offers specialized protein methacrylation analysis at both the targeted protein and proteomics levels. Our targeted protein methacrylation analysis uses advanced immunoenrichment techniques and high-resolution mass spectrometry to precisely map methacrylation sites on specific proteins, providing insights into their regulatory roles in gene expression and cellular functions. Meanwhile, our methacrylation proteomics employs high-throughput mass spectrometry to analyze methacrylation modifications on a global proteome scale, revealing comprehensive modification patterns. These services provide clients with detailed modification maps, facilitating the exploration of regulatory networks and driving scientific breakthroughs.
Service Advantages
Why Choose MtoZ Biolabs?
1. Advance Analysis Platform: MtoZ Biolabs established an advanced protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate PTM proteomics analysis data providing clients with a comprehensive data report.
4. Accurate Isomer Differentiation: Our technology effectively distinguishes between methacrylation (Kmea) and crotonylation (Kcr), despite their identical mass shifts.
5. Flexible Quantification Approaches: We offer both TMT/iTRAQ labeling methods and Label-Free quantification to meet a wide range of experimental requirements.
6. Comprehensive Site Mapping: Identification of multiple modification sites concurrently, generating a complete protein modification landscape.
Sample Submission Requirements
For more sample details, please consult our technical team.
Applications
Our protein methacrylation analysis services are applicable across a variety of research domains, including:
Deliverables
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs is dedicated to offering researchers precise and reliable PTM analysis services. Our state-of-the-art technology platforms and extensive expertise ensure comprehensive data support, driving innovation in scientific research. For further information, please contact us.
How to order?
