Protein Methylation Modification Sequencing

Protein methylation modification sequencing is a biotechnological method used to detect and analyze methylation modifications on proteins. This modification typically involves the addition of a methyl group (-CH3) to a specific amino acid residue on the protein, which plays a crucial role in regulating protein function and cellular processes.

 

Analysis Workflow

1. Sample Preparation

Proteins are extracted from biological samples.

 

2. Enzymatic Digestion

Proteins are cut into shorter fragments using enzymes (such as trypsin) to facilitate subsequent analysis.

 

3. Affinity Purification

Antibodies specific to methylation sites are used to enrich methylated proteins or peptides.

 

4. Mass Spectrometry

Purified peptides are measured using a mass spectrometer to identify the location and extent of methylation.

 

5. Data Analysis

Mass spectrometry data is analyzed using bioinformatics tools to determine methylation sites and study their biological significance.

 

Protein methylation modification sequencing combines methods like affinity purification and mass spectrometry to accurately identify methylation sites, helping to understand protein function regulation, signal transduction pathways, and protein expression changes under disease conditions. Its advantage lies in its ability to provide accurate modification maps, but the downside is that the equipment is costly, the technique is complex, and there are certain requirements for the amount of sample. With the advancement of technology, its analytical capabilities and application range are continuously expanding, especially important in fields like cancer research, neuroscience, and epigenetics.