Protein N-Glycosylation Analysis

Post-translational modifications (PTMs) of proteins encompass the chemical modifications that take place during or after the translation of proteins. These modifications are crucial in various cellular processes, including cell differentiation, protein degradation, signal transduction, regulatory processes, gene expression modulation, and protein interactions. One significant form of PTM is N-glycosylation, wherein sugar chains are attached to proteins via the nitrogen atom of amino acid residues, predominantly asparagine, following protein synthesis. This modification is widely observed in eukaryotic organisms and holds substantial biological significance. Comprehensive research on protein N-glycosylation (target protein N-glycosylation analysis and N-glycosylation proteomics) not only enhances our understanding of fundamental biological mechanisms but also opens new avenues for clinical diagnostics, drug development, and personalized medicine approaches.

 

Services at MtoZ Biolabs

MtoZ Biolabs utilizes Thermo Fisher's Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometry platforms, in combination with Nano-LC, to offer a comprehensive solution for protein N-glycosylation analysis, which include target protein N-glycosylation analysis and N-glycosylation proteomics. Simply provide us with your experimental objectives and sample materials, and we will manage the entire workflow, delivering detailed identification and quantification of N-glycosylation modifications on specific proteins and a panoramic view of N-glycosylation modifications across various samples, uncovering extensive N-glycosylation patterns. If you are interested in our protein N-glycosylation analysis service, please feel free to contact us. We are dedicated to providing you with the best service.

 

Analysis Workflow

 



 

Service Advantages

1. Advance Analysis Platform: Mtoz Biolabs established an advanced protein N-glycosylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein N-glycosylation analysis data providing clients with acomprehensive data report.

 

4. Comprehensive Analysis: Both target protein N-glycosylation analysis and N-glycosylation proteomics can be conducted in MtoZ Biolabs.

 

Sample Submission Requirements

 



1. If you are providing tissue samples, please ship them to us on dry ice.

 

2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.

 

3. Sample transportation: Please ensure that samples are shipped with sufficient dry ice and choose the fastest possible shipping way to minimize the risk of sample degradation during transit.

 

4. Before proceeding with the formal experiment, we will test the samples you provide, and the formal analysis will begin once the samples pass the quality check.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. The Detailed Information of Protein N-Glycosylation Analysis

4. Mass Spectrometry Image

5. Bioinformatics Analysis

6. Raw Data