Protein Network Analysis Service

Protein network analysis is a powerful technique that uncovers the vital roles of proteins in cellular functions, life processes, and disease mechanisms through the analysis of their interactions within the organism. Unlike traditional single-protein function analysis, which focuses on individual proteins, protein network analysis emphasizes the interactions and regulatory networks between proteins. It aims to provide a comprehensive understanding of how proteins collaborate to maintain the normal functions of an organism. This type of analysis offers researchers valuable insights into cellular functions, disease mechanisms, metabolic pathways, and more.

 

Protein network analysis extends beyond protein-protein interactions (i.e., protein interaction networks) to include interactions between proteins and other biomolecules, such as RNA, DNA, and metabolites. By examining networks across multiple levels and dimensions, researchers can reveal the complex factors that influence both health and disease in biological systems.

 



Too, I. H. K. et al. PLOS Pathog. 2018.

Figure 1. Protein Network Analysis

 

MtoZ Biolabs provides efficient and comprehensive Protein Network Analysis Service that helps researchers identify key nodes and regulatory mechanisms within protein networks, driving breakthroughs in basic research, disease studies, and drug development.

 

Services at MtoZ Biolabs

Protein network analysis builds complex network structures based on protein interactions and their broad connectivity within cells. By leveraging techniques like mass spectrometry, immunoprecipitation, western blotting (WB), and integrating databases and computational models, we help identify the roles of proteins in cellular processes. Our analysis platform incorporates advanced algorithms and tools (such as Cytoscape and Gephi) to analyze network structures, identify key proteins and modules, and explore their involvement in disease mechanisms. This network analysis helps elucidate how proteins collaborate within cells, offering a deeper understanding of the intricate mechanisms of biological processes.

 

MtoZ Biolabs’ Protein Network Analysis Service offers a full range of solutions, enabling comprehensive analysis of protein networks:

1. Protein Network Construction

Using mass spectrometry data and public databases, we assist in constructing protein networks in microorganisms, cells, or tissues, uncovering both direct and indirect protein interactions.

 

2. Functional Enrichment Analysis

We perform enrichment analysis on functional modules within protein networks, identifying key proteins and their roles in biological processes such as cell signaling, metabolism, and gene expression.

 

3. Network Module Analysis

We identify functional modules within protein networks and analyze their significance in biological processes, highlighting changes observed in health and disease states.

 

4. Disease Mechanism Analysis

Our protein network analysis helps identify proteins and regulatory pathways associated with specific diseases, providing critical insights into the molecular mechanisms underlying diseases.

 

5. Drug Target Identification

By analyzing protein networks, we identify potential drug targets, helping researchers uncover disease-related key proteins to facilitate drug discovery and development.

 

6. Quantitative and Dynamic Change Analysis

By combining quantitative mass spectrometry techniques, we examine dynamic changes in protein networks under different conditions, shedding light on how these networks are regulated in both physiological and pathological contexts.

 

Why Choose MtoZ Biolabs?

1. Efficient and Fast: We deliver rapid protein network construction and analysis, making our service ideal for time-sensitive applications like clinical emergencies and environmental monitoring.

2. Tailored Solutions: We provide highly customizable analysis plans to meet the specific needs and goals of each client, supporting research across various fields, from basic science to clinical applications.

3. Versatile Applicability: Whether for fundamental research, disease mechanism studies, or drug development, our Protein Network Analysis Service offers comprehensive support for your scientific endeavors.

4. Clear Visualization and Reporting: Through intuitive visualizations, such as network graphs and functional annotations, we present complex results in an easily understandable format, enabling quick identification of key research insights.

5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

Sample Submission Suggestions

1. Sample Types

Serum, plasma, urine, tissue extracts, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Preservation

Store samples at -20°C or lower to prevent degradation, avoiding repeated freeze-thaw cycles.

 

Note: Provide details on sample collection and handling.

 

Case Study

Case 1: Protein Network Analysis Identifies SARS-CoV-2 Enzyme Substrates

This study utilized substrate phage display and protein network analysis to identify the substrates of SARS-CoV-2 PLpro and 3CLpro enzymes in the human proteome. By constructing protein interaction networks, the study revealed the potential roles and interactions of these viral enzymes within host cells, providing valuable insights into the pathogenesis of COVID-19. This case illustrates the application of Protein Network Analysis Service in viral research, protein interactions, and drug target discovery, underscoring its significance in disease mechanism exploration and drug development.

 



Zhao, K. et al. JBC. 2023.

 

MtoZ Biolabs offers precise and efficient Protein Network Analysis Service that help you explore the roles of proteins in biological processes and uncover critical insights into disease mechanisms, drug targets, and functional modules. Contact us for professional technical support and high-quality service to advance your research and clinical applications.