Protein Nitrosylation Analysis
Post-translational modifications (PTMs) are chemical changes that occur to proteins during or after translation. These modifications are essential for various cellular processes, including cell differentiation, protein degradation, signal transduction, gene expression regulation, and protein interactions. One significant type of PTM is protein nitrosylation, which involves the addition of nitrosyl groups to specific amino acids, influencing the protein's structure and functionality. This modification is crucial for processes such as cellular signaling, responses to oxidative stress, and the regulation of protein activity. Exploring protein nitrosylation (target protein nitrosylation analysis and nitrosylation proteomics) in depth enhances our understanding of cellular biology and could provide insights into disease progression and potential therapeutic targets.
Services at MtoZ Biolabs
1. Target Protein Nitrosylation Analysis
MtoZ Biolabs provides Target Protein Nitrosylation Analysis focused on the identification and characterization of S-nitrosylation modifications on specific proteins. By integrating the Biotin Switch chemical derivatization method with immunoprecipitation (Co-IP) and high-resolution LC–MS/MS analysis, we specifically capture and detect nitrosylated cysteine residues, enabling precise localization and quantitative assessment of modification sites. This service is ideal for investigating the regulatory roles of S-nitrosylation on target proteins and supports studies related to signal transduction, redox regulation, and disease mechanisms.
2. Nitrosylation Proteomics Analysis
MtoZ Biolabs' Nitrosylation Proteomics Analysis utilizes a high-sensitivity mass spectrometry platform combined with the Biotin Switch enrichment strategy to systematically identify and quantify S-nitrosylated proteins in cells, tissues, and biofluids. Through optimized labeling and enrichment workflows, we comprehensively profile nitrosylated proteins, mapping modification sites and analyzing their associations with metabolic and signaling pathways. Coupled with bioinformatics interpretation, this service provides valuable insights into oxidative stress regulation, cellular signaling, and the discovery of potential biomarkers and therapeutic targets.
If you are interested in our protein nitrosylation analysis service, please feel free to contact us. We are dedicated to providing you with the best service.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform: Mtoz Biolabs established an advanced protein nitrosylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, with no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein nitrosylation analysis data providing clients with acomprehensive data report.
4. Comprehensive Analysis: Both target protein nitrosylation analysis and nitrosylation proteomics can be conducted in MtoZ Biolabs.
Sample Submission Requirements
1. If you are providing tissue samples, please ship them to us on dry ice.
2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.
3. Sample transportation: Please ensure that samples are shipped with sufficient dry ice and choose the fastest possible shipping way to minimize the risk of sample degradation during transit.
4. Before proceeding with the formal experiment, we will test the samples you provide, and the formal analysis will begin once the samples pass the quality check.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. The Detailed Information of Protein Nitrosylation Analysis
4. Mass Spectrometry Image
5. Bioinformatics Analysis
6. Raw Data
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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