Protein Omics Sequencing Cellular Sample Processing

The main steps in processing cell samples in proteomics include the following points:

 

Cell Culturing and Collection

Cells need to be cultured under appropriate conditions. When the cells reach an appropriate growth stage, they are collected through methods like centrifugation.

 

Cell Lysis

The collected cells are lysed to release their proteins through physical methods (such as ultrasound) or chemical methods (such as using lysis buffer).

 

Protein Extraction and Purification

After lysis, proteins are extracted and purified through methods like centrifugation and filtration.

 

Protein Identification and Quantification

The extracted and purified proteins can be identified and quantified through methods such as mass spectrometry and immunoblotting.

 

Data Analysis

Bioinformatics methods are used to analyze the identification and quantification results of the proteins.