Protein-Protein Interaction Sequencing

In-depth research on protein interaction plays a crucial role in life science research. Proteins are the main bearers of life activities, and their interactions form a complex molecular network within biological cells. Protein-Protein Interaction Sequencing (PPI-Seq) is an emerging technology. Based on high-throughput sequencing, it allows researchers to explore protein interactions at the whole genome level.

 

Technical Principles

The basic principle of Protein-Protein Interaction Sequencing is to use the yeast two-hybrid system or mammalian cell lines to express two proteins, then observe whether they interact. This interaction is detected qualitatively or quantitatively through the fluorescent intensity of the protein fusion tag. At the same time, with high-throughput sequencing technology, protein interaction relationships can be explored at the whole genome level.

 

Applications

PPI-Seq is instrumental in elucidating various biological processes within cells, such as signal transduction, gene regulation, and material transport. By studying the protein interaction network, it reveals the molecular mechanisms of various physiological and pathological processes in cells, providing new ideas for disease diagnosis and treatment. Also, Protein-Protein Interaction Sequencing can be applied to drug target screening and effectiveness evaluation. By studying protein interactions, new drug targets can be discovered, further facilitating drug screening and efficacy evaluation.

 

Although Protein-Protein Interaction Sequencing has extensive applications in life science research, this technology still presents some challenges, such as the dynamics of protein interaction, subcellular localization, and protein modification. With the continuous advancement of sequencing technology, we are confident that we can solve these problems and achieve more in-depth and detailed research on protein interaction networks. In general, as a novel biological research method, Protein-Protein Interaction Sequencing, with its unique advantages and broad application prospects, is gradually attracting attention from the scientific community.