Protein Thermal Stability Detection Methods

Protein thermal stability testing is an important method for assessing the stability of proteins at different temperatures, mainly used for studying the relationship between protein structure and function.

 

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1. Differential Scanning Calorimetry (DSC)

DSC is a commonly used thermal analysis technique for measuring the thermal stability of proteins during the heating process. This method determines the thermal denaturation temperature (Tm) of the protein by comparing the difference in heat absorption or release between the sample and the reference during the heating process.

 

2. Circular Dichroism (CD)

CD can be used to monitor changes in the secondary structure of proteins. By measuring the changes in the circular dichroism signal of the protein at different temperatures, the thermal stability of the protein can be assessed.

 

3. Fluorescence Spectroscopy

The tryptophan and tyrosine residues in proteins have different fluorescence characteristics in different structural environments. By measuring the changes in the fluorescence intensity of these residues at different temperatures, the thermal stability of the protein can be inferred.

 

4. Surface Plasmon Resonance (SPR)

SPR can be used to monitor the binding characteristics of proteins at different temperatures, thereby assessing their thermal stability.

 

In practical applications, a combination of multiple methods is often used to comprehensively evaluate the thermal stability of proteins.