Proteomic Analysis Service
Proteomic analysis service provides a comprehensive study of protein composition, structure, and function at a large scale, assisting researchers, medical institutions, and businesses in understanding proteomic data from biological samples. Focused on the holistic analysis of proteins within cells or tissues under specific conditions, proteomics enables insights into protein roles across biological processes, unveiling disease mechanisms, developing therapeutic strategies, and discovering biomarkers. In medicine, proteomics is pivotal for advancing precision medicine, facilitating early disease diagnosis and personalized treatment plans through the analysis of patient protein expression profiles. In agriculture, proteomic analysis service aids in identifying proteins linked to crop growth and disease resistance, enhancing yield and quality. In environmental science, proteomics assesses the ecological impact of pollution, supporting environmental conservation efforts. Importantly, proteomics reveals biological effects following gene expression, providing insights beyond the genomic blueprint by showing how genes translate into functional proteins within organisms.
Services at MtoZ Biolabs
1. Sample Processing
(1) Protein Extraction: Tailored extraction methods are employed based on sample type, such as cells, tissues, or fluids. For instance, cell samples may require lysis buffers with protease inhibitors, while tissue samples often need grinding before extraction.
(2) Protein Purification: Impurities like nucleic acids and lipids are removed to produce highly purified protein samples using techniques like salting out, ultrafiltration, and affinity chromatography.
(3) Protein Quantification: Accurate measurement of protein concentration is conducted using methods such as the Bradford or BCA assays, standardizing samples for subsequent analyses.
2. Protein Separation and Identification
(1) Two-Dimensional Electrophoresis (2-DE): Proteins are separated based on isoelectric point and molecular weight using isoelectric focusing combined with SDS-PAGE, producing a two-dimensional protein map for comparative analysis.
(2) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): Central to protein identification, LC-MS/MS separates peptides derived from protein digestion, followed by mass spectrometry to determine mass-to-charge ratios and sequences, which are matched against protein databases.
(3) iTRAQ/TMT Labeling: Enables relative quantification of proteins across multiple samples by comparing signal intensities of labeled peptides in mass spectrometry analysis.
3. Post-Translational Modification Analysis
(1) Phosphorylation Analysis: Utilizes enrichment methods for phosphorylated peptides, such as TiO₂ affinity chromatography and IMAC, combined with mass spectrometry to determine phosphorylation sites and levels.
(2) Glycosylation Analysis: Different approaches are used for N-glycosylation and O-glycosylation, including enzymatic glycan release or chemical labeling, followed by mass spectrometry or chromatography.
(3) Other Modifications: Analysis of acetylation, methylation, and other modifications relies on targeted enrichment and mass spectrometry techniques.
4. Bioinformatics Analysis
(1) Protein Database Search: Mass spectrometry-derived peptide data are compared with databases like UniProt and NCBI to identify proteins.
(2) Differential Expression Analysis: Protein expression differences between samples (e.g., diseased vs. healthy) are assessed using statistical methods like t-tests and ANOVA.
(3) Protein Function Annotation and Pathway Analysis: Proteins are classified by function and mapped to biological pathways, such as KEGG and Reactome, to elucidate their roles in biological processes.
Proper sample handling and storage are critical in proteomic analysis service to prevent protein degradation, necessitating rapid low-temperature processing. The choice of databases and algorithms in data interpretation directly influences result accuracy. Challenges in proteomic analysis include sample complexity, detection of low-abundance proteins, and large data volumes requiring sophisticated analysis. Proteomic analysis service offers high sensitivity and broad coverage, enabling simultaneous analysis of thousands of proteins and providing comprehensive expression profiles. Integrating proteomics with other omics technologies like metabolomics and transcriptomics offers holistic solutions for systems biology research.
Advantages of MtoZ Biolabs Proteomic Analysis Service
1. State-of-the-Art Equipment and Expertise
MtoZ Biolabs hosts advanced proteomic platforms with equipment such as high-resolution mass spectrometers and high-efficiency liquid chromatographs. Our skilled technicians ensure data accuracy and reliability through expert experimental and analytical methodologies.
2. High Efficiency and Throughput
Our capability to process multiple samples concurrently enhances experimental efficiency. Automated sample handling and high-throughput mass spectrometry systems enable swift analysis of large sample sets.
3. Tailored Services
We offer customized service plans based on client-specific needs, including research goals, sample types, and analytical methods. For drug development clients, we focus on proteomic analysis service for drug target screening and mechanism of action studies.
MtoZ Biolabs is dedicated to providing premium proteomic analysis service tailored to diverse research requirements. With extensive experience and advanced analytical techniques, we support your research breakthroughs and project success. Partner with MtoZ Biolabs for expert technical support and personalized service, as we explore the intricacies of life sciences together.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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