Proteomics Analysis Strategy

Proteomics analysis includes analyzing the structure and function of proteins, post-translational modifications (PTMs), protein localization, protein expression, and the interactions among proteins, etc. Based on different analytical content, different proteomics analysis techniques and strategies can be adopted.

 

Mass spectrometry (MS) is one of the most commonly used techniques in proteomics analysis. Currently, proteomics analysis strategies based on MS mainly include bottom-up proteomics and top-down proteomics. In bottom-up proteomics, proteins need to be digested into peptides before mass spectrometric analysis, and this strategy is adopted by common label-free and labeling quantitative proteomics techniques. In top-down proteomics, protein are not digested but are directly analyzed, which can preserve most of the features of proteins with unstable structures that are mostly destroyed in the bottom-up strategy, and also reduces the time consumption of digestion. Researchers can choose the appropriate strategy for proteomics analysis according to different research needs.

 

MtoZ Biolabs provides various proteomics analysis services using the Thermo Fisher Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoscale chromatography.

 

Services at MtoZ Biolabs

1. Top-down Proteomics

2. DIA-PRM Proteomics

3. PCT-DIA Proteomics