Purity analysis SDS-PAGE

SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is a widely utilized electrophoresis technique for the separation and analysis of proteins. This method separates proteins based on their molecular weight by denaturing them in the presence of SDS and running them through a polyacrylamide gel. SDS-PAGE is extensively used for protein purity analysis, molecular weight determination, protein quantification, and structural studies. It has significant applications in biopharmaceuticals, protein research, and biochemical analysis.

 

MtoZ Biolabs offers professional SDS-PAGE purity analysis services, employing efficient experimental techniques and advanced image analysis software to provide clients with accurate and reliable protein purity results, supporting scientific research and applications.

 

Analysis Workflow

1. Sample Preparation

Denature protein samples by boiling them in SDS-containing sample buffer to ensure complete denaturation.

 

2. Gel Electrophoresis

Load the treated samples into a polyacrylamide gel and separate the proteins by electrophoresis.

 

3. Staining and Destaining

Use Coomassie Brilliant Blue or silver staining to visualize the protein bands on the gel.

 

4. Image Acquisition and Analysis

Scan and analyze the gel image using advanced image analysis software to calculate protein purity.

 



 

Service Advantages

 



 

Applications

Biopharmaceuticals: Analyze the purity of therapeutic proteins to ensure product quality and safety.

 

Protein Research: Evaluate the purity of protein samples to support subsequent structural and functional studies.

 

Biochemical Analysis: Monitor each step of protein purification to improve purification efficiency.

 

Quality Control: Conduct quality control during production to ensure the final product meets purity standards.

 

 

MtoZ Biolabs is committed to providing high-quality SDS-PAGE purity analysis services for scientific research and applications with an advanced technology platform and extensive project experience. For more information or consulting services, please contact our technical support team.