Quantitative Tyrosine Phosphoproteomics Analysis Service

Protein phosphorylation is a common post-translational modification in eukaryotes. Eukaryotic cell protein phosphorylation is divided into two types: serine (Ser)/threonine (Thr) phosphorylation and tyrosine (Tyr) phosphorylation. Although the proportion of Tyr phosphorylation is small, it plays an extremely important role in the fate of cells and individuals, and is closely related to diseases such as cancer. Studies have shown that Tyr kinase genes account for 75% of oncogenes, and 63% of protein kinase tumor-targeting drugs are also Tyr kinase inhibitors.

 

Quantitative proteomics analysis of Tyr phosphorylation is an efficient method for in-depth study of protein Tyr phosphorylation events in cell signal transduction processes. Its principle mainly involves precise qualitative and quantitative analysis of Tyr-phosphorylated proteins through technologies such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), revealing Tyr kinases related to major diseases such as cancer, and aiding the discovery of Tyr kinase inhibitors. In quantitative proteomics analysis of Tyr phosphorylation, the enrichment of Tyr-phosphorylated peptides is the most crucial step. Depending on experimental needs, MtoZ Biolabs can choose antibody affinity enrichment or SH2 superbinder enrichment methods to achieve efficient sample enrichment, and can achieve stepwise separation of mono-phosphorylated and multi-phosphorylated peptides, avoiding the impact of ionization efficiency issues of multi-phosphorylated peptides on the identification and quantification of mono-phosphorylated peptides during mass spectrometry analysis.

 



Figure 1. MtoZ Biolabs - Quantitative Proteomics Analysis Process of Tyrosine Phosphorylation

 

Based on Thermo's Obitrap Fusion Lumos mass spectrometer combined with Nano-LC technology, MtoZ Biolabs has established a quantitative proteomics analysis platform for Tyr phosphorylation. Whether you are engaged in basic research or biopharmaceutical development, our quantitative proteomics analysis service of Tyr phosphorylation will provide powerful support and accurate data for your research. Free project evaluation!

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with detailed technical information, including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Tyr Phosphorylation Analysis

4. Mass Spectrometry Images

5. Raw Data