Quantitative Proteomics Study of Cell Histone Methylation

In the genetics and functional regulation of cells, in addition to the DNA sequence, epigenetics also play a crucial role. Among them, protein methylation as a key modification method of epigenetics, has a decisive significance for regulating gene expression and cell fate. MtoZ Biolabs, based on cell histone methylation, has launched a new research platform for methylation quantitative proteomics, revealing the deep secrets of protein methylation for researchers around the world.

 

Sample Submission Requirements

Histones are proteins in the cell nucleus that bind to DNA. They are not only the "packing material" for DNA, but also key regulatory factors for cellular genetic information. Histones undergo various chemical modifications, such as methylation, acetylation, etc. These modifications play a crucial role in cell growth, differentiation, and function.

 

Utilizing Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nano-level chromatography, we offer methylation quantitative proteomics analysis services. By enriching the methylated peptide segments with specific antibodies targeting different methylation sites and modification forms, and combining them with LC-MS/MS analysis, we can achieve large-scale quantitative and qualitative analysis of methylated proteins. We accurately identify and quantify the methylation modifications of histones, revealing the mechanisms of cell biology and disease onset for researchers.

 

Service Advantages

1. Comprehensive Identification

Combined with the latest mass spectrometry technology, we can comprehensively identify the methylation sites of histones, whether they are mono, di, or tri-methylation.

 

2. High Sensitivity Quantification

By using isotope labeling technology, we can accurately quantify the methylation modifications of histones, ensuring the reliability and repeatability of the results.

 

3. In-Depth Data Analysis

Equipped with a professional bioinformatics team, we provide researchers with profound insights by comparing data under different samples or treatment conditions.

 

4. Strict Quality Control System

We implement strict quality control at every step, including sample processing, protein extraction, and mass spectrometric analysis, to ensure that each step reaches the optimal condition.

 

Applications

1. Epigenetic Regulation Mechanisms of Gene Expression

2. Differential Analysis of Protein Methylation Under Different Cell States or Physiological Environments

3. Screening and Identification of Methylation Sites Related to Disease Onset and Development

4. The Study of Srug Action Mechanisms and Their Relationship with Methylation Modifications

 

MtoZ Biolabs has accumulated rich experience and technical deposits in the field of methylation quantitative proteomics research. We are committed to providing the most advanced and efficient technical services to researchers around the world, helping epigenetic research stay at the forefront of scientific research. If you are conducting related research or are interested in this technology, please feel free to communicate with us at any time. We will provide you detailed technical introductions and service plans. MtoZ Biolabs looks forward to working with you. Free project evaluation,Welcome to learn more details!