Recombinant Protein Drug and Receptor Affinity Detection

Recombinant protein drugs are protein-based therapeutic drugs produced using DNA recombination technology or other biotechnologies, including cytokines, polypeptide hormones, recombinant enzymes, monoclonal antibodies, fusion proteins, etc. Compared with traditional low-molecular synthetic drugs, recombinant protein drugs have advantages such as strong specificity, low toxicity, and significant therapeutic effects.

 

Affinity is a characteristic parameter for evaluating the strength of the interaction between two molecules in a reversible reaction process. Testing the affinity between recombinant protein drugs and their receptors, evaluating their binding strength and stability, can determine the interaction characteristics between the drug and the receptor, which is crucial for the drug discovery and development process. For example, in the process of drug optimization, affinity information can guide the modification and optimization of the structure of recombinant protein drugs to improve their binding with receptors. In addition to guiding early drug research and development, screening and identification, the detection of affinity between recombinant protein drugs and receptors can also be applied to preclinical and clinical research and downstream production quality control.

 

The methods for detecting the affinity between recombinant protein drugs and their receptors include Surface Plasmon Resonance (SPR), Immunoprecipitation (IP), SPR combined with Mass Spectrometry (SPR-MS), and Bio-Layer Interferometry (BLI). Among them, SPR and BLI are commonly used, while SPR-MS can monitor the binding of protein drugs and receptors in real time, and determine the mass of the binding complex through mass spectrometry analysis.

 

MtoZ Biolabs provides professional affinity detection services for recombinant protein drugs and receptors based on Surface Plasmon Resonance technology, high-resolution mass spectrometry technology, and efficient chromatographic separation technology. In addition, MtoZ Biolabs also provides a variety of biological product characterization services, such as structural characterization, charge heterogeneity characterization, and impurity analysis. Please feel free to inquire.