Resources

Proteomics Databases



Metabolomics Databases

• • Protein Isoelectric Point Analysis in Adipose Tissue via CIEF

  Adipose tissue, as the main energy storage organ in the human body, plays multiple physiological roles including energy storage, hormone secretion, and regulation of overall metabolism. In recent years, with the increasing prevalence of obesity and related metabolic disorders, proteomic analysis of adipose tissue has become a hot research topic.

• • Analysis of Protein Content in Liver Tissue

  The liver, as the largest solid organ in the human body, carries out complex physiological functions. It is primarily involved in blood purification and detoxification, metabolic regulation, protein synthesis, and bile production. Protein plays a crucial role in the functions of the liver. The liver is responsible for synthesizing various plasma proteins (such as albumin, clotting factors, components of the complement system), as well as being involved in the production and metabolism of lipoproteins.

• • Fish Tissue Protein Glycan Profiling Detection

  Fish tissue protein is the main component of fish meat, including various amino acids and proteins, which has important value for human health. These proteins not only determine the nutritional quality of fish meat, but also affect its taste and processing characteristics. The protein-glycan spectrum detection technology of fish tissue can comprehensively evaluate the quality and characteristics of fish meat.

• • Mechanism of 2D-Electrophoresis

  Two-Dimensional Electrophoresis (2-DE) is a widely utilized separation technique in proteomics research. By leveraging the differences in proteins' isoelectric points (pI) and molecular weights, 2-DE achieves high-resolution separation of complex protein mixtures through two sequential, orthogonal electrophoresis processes.

• • Application of 2D-Electrophoresis

  Two-Dimensional Electrophoresis (2-DE) is a crucial technique extensively used in proteomics research. It allows the separation of complex protein mixtures, and when combined with mass spectrometry, it facilitates the identification and quantification of proteins.

• • Advantages and Disadvantages of 2D-Electrophoresis

  Two-Dimensional Electrophoresis (2-DE) is a widely used technique in proteomics research. This method separates protein mixtures first by isoelectric focusing (IEF) and then by SDS-PAGE, achieving high-resolution analysis of complex protein samples.

• • Application of 1D SDS-PAGE and IEF

  1D SDS-PAGE (one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and isoelectric focusing (IEF) are widely used techniques in proteomics research. Each of these methods has unique advantages and applications, making them essential tools for protein analysis, identification, and research. This article provides an in-depth introduction to the principles, applications, and significance of these two techniques in modern biological research.

• • 1D SDS-PAGE and IEF Mechanism

  In the field of molecular biology and biochemistry, protein separation techniques are indispensable tools. Two commonly used methods are one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE) and isoelectric focusing (IEF). This article will delve into the mechanisms of these two techniques.

• • Advantages and Disadvantages of 1D SDS-PAGE and IEF

  In the field of protein analysis and separation, 1D SDS-PAGE (one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and IEF (isoelectric focusing) are two commonly used electrophoretic techniques. They play a critical role in protein identification, purification, and quantitative analysis. This article will explore the advantages and disadvantages of these two methods to assist researchers in choosing the appropriate technique for their experiments.

• • Mechanism of SDS-PAGE in Protein Separation

  Proteins are the molecular machines that perform a variety of functions within biological organisms. Understanding protein structure and function is essential for elucidating life processes and disease mechanisms. Protein separation is a critical step in protein research, aiding scientists in the analysis and purification of specific proteins. SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) is a widely employed technique for protein separation in laboratories.