Sample Preparation and Submission Guide for Proteomics Research

The submission of samples in proteomics research needs to follow certain guidelines and steps to ensure the quality of the samples and the accuracy of the analysis results. The following are general guidelines for preparing and submitting samples:

 

1. Sample Preparation

(1) Ensure the representativeness and sufficient quantity of the sample.

(2) Use appropriate buffers and inhibitors to extract proteins, avoiding protein degradation and modification.

(3) Remove cell debris and undissolved material through centrifugation or filtration.

(4) Quantify protein and evaluate purity (such as through the BCA Protein Assay, SDS-PAGE, etc.).

 

2. Sample Storage and Transportation

(1) Freeze samples according to the type of sample (usually -80°C).

(2) Ensure the cold chain of the sample during transportation by using dry ice or ice packs.

 

3. Sample Labeling and Recording

(1) Clearly label samples, including sample number, collection date, sample type, etc.

(2) Record in detail the sample handling process and any conditions that may affect the experimental results.

 

4. Communication with the Laboratory

(1) Communicate with the laboratory before submitting samples to understand specific sample requirements and analysis methods.

(2) Provide necessary background information and experimental design to help the lab better understand the research purpose and needs.

 

5. Transportation and Sample Submission

(1) Follow the specific instructions provided by the laboratory for sample packaging and transportation.

(2) Ensure the safety and integrity of the sample during transportation.