Single Cell Proteomics Service

Single cell proteomics is a cutting-edge technology focused on analyzing protein expression, modifications, and functional states at the single cell level. Utilizing highly sensitive mass spectrometry or other detection methods, it enables comprehensive characterization of the proteome within individual cells. Single cell proteomics reveals the molecular characteristics, dynamic changes, and specific roles of individual cells within biological systems. Single cell proteomics includes two main stages: sample preparation and mass spectrometry detection. During sample preparation, individual cells are isolated, and proteins are extracted through surface contact, protein separation, and peptide preparation steps. As protein and peptide loss can occur during this process, strict control of operating conditions is required to maximize sample retention. Subsequently, peptides are ionized and analyzed using mass spectrometry. The detection stage offers two approaches: label-free analysis and multiplexed analysis. Label-free analysis typically processes 40 cells per day, providing direct quantitative and sequence information. Multiplexed analysis, incorporating up to 18 tags, significantly increases throughput, processing up to 256 cells per day while simultaneously obtaining quantitative and sequence data.

 



Bennett, HM. et al. Nat Methods. 2023.

Figure 1. The Workflow of Single Cell Proteomics

 

Single cell proteomics helps decipher complex intracellular signaling and regulatory mechanisms, identify the functional characteristics of specific cell subpopulations, and discover molecular markers related to disease progression. Furthermore, single cell proteomics effectively addresses cellular heterogeneity, revealing the distinct features of different cell types or states within tissues, and provides precise molecular data for basic research, disease diagnosis, and drug development.

 

Services at MtoZ Biolabs

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. MtoZ Biolabs offers single cell proteomics service focusing on in-depth analysis of protein expression, modifications, and functional states at the single cell level. Using advanced mass spectrometry platforms and optimized sample preparation workflows, we efficiently extract proteins from individual cells for precise analysis. Our service supports various analytical modes, including high-throughput multiplexed analysis and flexible label-free analysis, providing both quantitative and sequence information tailored to research needs. With customized data processing methods, we delve into single cell proteomics data to uncover cellular heterogeneity, analyze complex signaling pathways, and identify disease-associated molecular features. Whether for basic research or drug development, our single cell proteomics service delivers high-quality, reliable solutions to meet diverse scientific requirements. If yu are interested in our service, please contact us freely.

 

Service Advantages

1. High Sensitivity and Resolution  

MtoZ Biolabs utilizes highly sensitive mass spectrometry technologies to achieve precise protein detection at the single cell level, overcoming the limitations of traditional antibody-based methods. Our single cell proteomics service provides reliable support for the comprehensive analysis of low-abundance proteins, post-translational modifications (PTMs), and protein interactions.  

 

2. High Throughput and Multiplexing  

With enhanced multiplexing capacity and throughput, MtoZ Biolabs’ single cell proteomics service generates high-dimensional single cell datasets. By leveraging advanced data analysis tools, it extracts critical biological insights, facilitating the elucidation of dynamic relationships between signaling pathways and cellular functions.  

 

3. Multi-Omics Integration  

Our single cell proteomics service integrates single cell protein data with genomics, transcriptomics, and metabolomics datasets, providing a comprehensive multi-omics analysis framework. This enables the exploration of complex biological networks at the single cell level and connects cellular heterogeneity to its biological context.  

 

4. Customized Data Analysis  

Our single cell proteomics service offers specially designed bioinformatics tools to align experimental data with specific research questions. It enables in-depth exploration of signal fluctuations and protein-protein correlations within single cell proteomics data, assisting in predicting the effects of environmental or drug perturbations on cellular signaling states.

 

Case Study

1. High-throughput And Scalable Single Cell Proteomics Identifies Over 5000 Proteins Per Cell

 



Ye, Z. et al. bioRxiv. 2023.

Figure 2. Workflow and Results of High-Throughput Label-Free Single Cell Proteomics

 

2. Quantitative Single cell Proteomics As A Tool to Characterize Cellular Hierarchies

 



Schoof, EM. et al. Nat Commun. 2021.

Figure 3. Detection of Cellular Heterogeneity with Single Cell Proteomics

 

3. Single cell Proteomics Delineates Murine Systemic Immune Response To Blast Lung Injury

 



Li, L. et al. Commun Biol. 2024.

Figure 4. Immune landscape in the Spleen (SP) in the Acute Phase After Blast

 

4. Single cell Proteomics Defines The Cellular Heterogeneity of Localized Prostate Cancer

 



Jacobs, A. et al. Cell Rep Med. 2022.

Figure 5. Analysis of Prostate Cancer Samples by Single Cell Proteomics

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Single Cell Proteomics

5. Mass Spectrometry Image

6. Raw Data