Single-Cell RNA Sequencing Service
- Reveal cellular uniqueness and discover new targets and biomarkers.
- Explore cellular heterogeneity.
- Study differentiation and development at the cellular level.
- Track disease and therapeutic progress at the cellular level.
- Identify new cell populations.
Single-cell RNA sequencing is a groundbreaking high-throughput gene expression analysis technology that reveals gene expression differences, cell type characteristics, and dynamic changes in cell states at the single-cell level. Unlike traditional bulk RNA sequencing, single-cell RNA sequencing avoids the influence of population-level averaging effects, captures subtle differences between cells, uncovers heterogeneity, and provides an in-depth analysis of the functional state of each cell in a biological system.
Widely applied in tumor microenvironment studies, developmental biology, immune system research, and disease exploration, single-cell RNA sequencing has become a fundamental tool in modern biology. MtoZ Biolabs offers comprehensive, end-to-end Single-Cell RNA Sequencing Service utilizing the 10x Genomics Chromium system in combination with Illumina sequencing platforms. This service covers all aspects, including cell sorting, library construction, sequencing, and data analysis, ensuring accurate and high-quality results.
Technical Principles
The 10x Genomics Chromium system is a core technological platform for Single-Cell RNA Sequencing Service. Through microfluidic technology, individual cells are encapsulated with gel beads labeled with unique barcodes (10x barcoded gel beads) into Gel Bead-in-Emulsions (GEMs). Each droplet acts as an independent micro-reactor, ensuring that the mRNA of each cell can be uniquely labeled. Within the droplets, cells undergo lysis, releasing mRNA, followed by reverse transcription and library construction under barcode labeling. Subsequently, the barcoded cDNA library undergoes deep sequencing using the Illumina high-throughput sequencing platform. Finally, bioinformatics tools are employed to analyze cell types, gene expression patterns, and developmental trajectories. This process effectively eliminates population-level averaging effects, accurately revealing cellular heterogeneity.
Hume, A. J. et al. Pathogens. 2024.
Technical Advantages
1. High Throughput: Capture thousands to tens of thousands of single cells in one run, revealing population heterogeneity and rare cell groups.
2. High Precision: Cellular barcodes and Unique Molecular Identifiers (UMIs) ensure data fidelity and reduce amplification bias.
3. High Sensitivity: Detect low-abundance gene expression and capture subtle transcription signals within cells.
4. Reproducibility: Highly standardized operational procedures ensure experimental reproducibility and data stability.
Analysis Workflow
The Single-Cell RNA Sequencing Service from MtoZ Biolabs provides a full-process one-stop service from tissue samples to data analysis, ensuring that every step undergoes strict quality control to maximize the retention of single-cell biological information.
1. Sample Reception and Processing
Receive fresh or frozen tissue samples, perform tissue dissociation, and obtain high-quality single-cell suspension, ensuring a cell viability rate >85%.
2. Single-Cell Isolation and Barcode Labeling
Use the 10x Genomics Chromium system to encapsulate individual cells with gel beads labeled with unique barcodes, ensuring that the mRNA of each cell is uniquely labeled.
3. Reverse Transcription and Library Construction
Perform cell lysis and mRNA reverse transcription within droplets, construct barcoded cDNA libraries, and amplify them.
4. High-Throughput Sequencing
Perform high-depth sequencing using the Illumina NovaSeq platform to capture single-cell gene expression information.
5. Data Analysis
Perform data analysis, including cell subgroup classification, differential gene expression analysis, and developmental trajectory reconstruction.
Cai, C. C. et al. Biomed. Pharmacother. 2023.
Why Choose MtoZ Biolabs?
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced single-cell RNA sequencing platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all single-cell RNA sequences data, providing clients with a comprehensive data report.
Applications
MtoZ Biolabs’ Single-Cell RNA Sequencing Service provides advanced tools to explore gene expression at the individual cell level, offering deeper insights into cellular processes and molecular mechanisms. This service enables you to:
Case Study
Case 1: Single-Cell RNA Sequencing Analysis of Skeletal Muscle Development Based on 10x Genomics Chromium
This study used the 10x Genomics Chromium platform for single-cell RNA sequencing to deeply analyze cellular heterogeneity and dynamic gene expression during skeletal muscle development. The study constructed a cell differentiation trajectory map, identified key regulatory genes and signaling networks, and provided critical data support for research on muscle development mechanisms and related diseases.
Cai, C. C. et al. Biomed. Pharmacother. 2023.
Case 2: Single-Cell RNA Sequencing Analysis of Environmental Gene Dynamics in PDL Cells
This study utilized single-cell RNA sequencing technology to analyze gene expression changes in human periodontal ligament (PDL) cells under in vivo and in vitro conditions. The results revealed the impact of environmental conditions on gene regulation and cellular function in PDL cells, providing key data support for tissue repair and regeneration research, highlighting the application value of single-cell RNA sequencing in studying cellular function and environmental adaptability.
Abdallah, A. T. et al. Int. J. Mol. Sci. 2024.
FAQ
Q1: What is the difference between RNA sequencing and single-cell RNA sequencing?
RNA sequencing is a technology that sequences all RNA in a bulk sample and is suitable for analyzing large-scale RNA expression data, typically used for RNA analysis in tissues or cell populations. In contrast, single-cell RNA sequencing sequences RNA at the single-cell level, revealing heterogeneity in gene expression among different cells and providing higher-resolution gene expression information.
MtoZ Biolabs is committed to providing high-quality Single-Cell RNA Sequencing Service for researchers worldwide. With advanced technological platforms and a professional team, we help you precisely analyze molecular mechanisms at the single-cell level, driving innovation and breakthroughs in scientific research.
Choose MtoZ Biolabs, and let single-cell gene expression data provide precise support for your research projects!
How to order?
