Single Nucleus RNA Sequencing Service

Single nucleus RNA sequencing (snRNA-seq) is an advanced transcriptomic technology designed to profile gene expression at the single-nucleus level, especially in tissues where isolating intact cells is challenging. Unlike traditional single-cell RNA sequencing, single nucleus RNA sequencing extracts RNA directly from isolated nuclei, enabling transcriptomic analysis from frozen tissues, archived samples, and structurally complex tissues. Single nucleus RNA sequencing method preserves the spatial and molecular integrity of cellular content, offering an accurate representation of gene expression profiles across diverse biological contexts. MtoZ Biolabs delivers Single Nucleus RNA Sequencing Service with precision and reliability, empowering researchers to explore cellular heterogeneity and transcriptional dynamics across various experimental models. 

 

The Single Nucleus RNA Sequencing Service plays a critical role in uncovering cellular heterogeneity, identifying rare cell populations, and elucidating gene regulatory networks in complex tissues. It is particularly useful in studies where intact single cells cannot be efficiently isolated, such as frozen brain tissue, fibrotic organs, or certain tumor samples. Single nucleus RNA sequencing minimizes technical artifacts and enhances transcriptomic resolution by directly analyzing nuclear RNA. As a versatile tool, single nucleus RNA sequencing technology is widely applied in fields such as neuroscience, oncology, developmental biology, and immunology.

 

Service at MtoZ Biolabs

MtoZ Biolabs leverages state-of-the-art technologies and bioinformatics pipelines to ensure high-resolution transcriptomic data that aligns with cutting-edge research standards. MtoZ Biolabs' Single Nucleus RNA Sequencing Service offers a comprehensive workflow that includes sample preparation, nuclei isolation, RNA extraction, library construction, high-throughput sequencing, and sophisticated bioinformatics analysis. Our team of experienced scientists ensures each step is meticulously executed to deliver reliable and reproducible results. Researchers can rely on our services to gain unparalleled insights into gene expression patterns, cellular interactions, and molecular mechanisms underlying various biological processes.

 



Piwecka, M. et al. Nat Rev Neurol. 2023.

 

Figure 1. Experimental Pipeline of a Droplet-Based Single Nucleus RNA Sequencing in a Nutshell

 

Service Advantages

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Single Nucleus RNA Sequencing Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. Preservation of Tissue Integrity: By isolating RNA directly from nuclei, the Single Nucleus RNA Sequencing Service minimizes dissociation-induced artifacts and preserves the native transcriptomic state of cells. This is particularly advantageous for studying highly structured or complex tissues like brain or tumor microenvironments.

3. Customized Bioinformatics Analysis: Our expert bioinformatics team delivers tailored data analysis, including differential gene expression, clustering, pathway enrichment, and advanced visualization, to meet the specific needs of diverse research objectives.

4. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

5. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Single Nucleus RNA Sequencing Service data, providing clients with a comprehensive data report.

6. Broad Research Applications: The Single Nucleus RNA Sequencing Service supports a wide range of research areas, including oncology, neuroscience, immunology, and developmental biology, making it a versatile tool for uncovering cellular heterogeneity, identifying rare cell populations, and exploring transcriptional dynamics.

 

Case Study

Unveiling Cell-Type Specific Vulnerability in Alzheimer’s Disease Using Single Nucleus RNA Sequencing

Using single nucleus RNA sequencing, researchers investigated the selective vulnerability of neuronal populations in Alzheimer’s disease (AD) across different disease stages. Brain samples from the entorhinal cortex (EC) and superior frontal gyrus (SFG), regions affected early and late in AD progression, were analyzed across Braak stages (0, 2, 6). Nuclei were isolated from frozen tissues, sequenced, and subjected to detailed bioinformatics analysis. As shown in the image, t-SNE projections illustrate distinct cell-type clusters in the EC and SFG, revealing dynamic shifts in cell composition across disease stages. Heatmaps display marker gene expression patterns, highlighting changes in neuronal and glial subpopulations. Bar graphs quantify the average number of genes detected per cell and the abundance of major cell types at each disease stage. The study identified RORB+ excitatory neurons in the EC as highly susceptible to tau pathology and a reactive astrocyte subpopulation with reduced homeostatic gene expression. These findings underscore the power of single nucleus RNA sequencing in decoding cell-type-specific transcriptional changes, advancing our understanding of AD pathology, and identifying potential therapeutic targets. High-resolution Single Nucleus RNA Sequencing Services empower researchers to unravel complex transcriptomic landscapes in neurodegenerative diseases.

 



 

Leng, K. et al. Nat Neurosci. 2021.

 

Figure 2. AD Progression Differentially Affects the Cell-Type Composition of the EC and SFG

 

FAQ

Q: What are the specific requirements for sample preservation and storage before single nucleus RNA sequencing?

For Single Nucleus RNA Sequencing, tissue samples must be snap-frozen immediately in liquid nitrogen or on dry ice to prevent RNA degradation. Store samples at -80°C, avoiding freeze-thaw cycles. Samples should remain RNase-free and transported on dry ice to maintain stability. Specific tissues may require tailored pre-processing protocols. Proper preservation ensures nuclei integrity and RNA quality, critical for reliable sequencing outcomes. For special requirements, please contact MtoZ Biolabs for customized guidance.

 

Our Single Nucleus RNA Sequencing Service is designed to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.