Single-Protoplast Proteomics Analysis

Single protoplasts are cells from plants, fungi, or bacteria that have had their cell walls removed through enzymatic or mechanical processes. These cells are widely used in research due to their ability to directly reflect internal cellular changes, playing a vital role in studies of cell differentiation, signal transduction, and metabolic regulation. Traditional proteomics approaches often require large sample sizes, making it challenging to analyze single cells or small numbers of cells with sufficient sensitivity. To overcome this, Single-Protoplast Proteomics Analysis has emerged, enabling high-sensitivity proteomics data to be acquired from single cells, revealing dynamic protein expression profiles at the single-cell level.

 



Xu Y, et al. Trends Plant Sci. 2022.

 

Services at MtoZ Biolabs

MtoZ Biolabs provides advanced proteomics analysis solutions with our state-of-the-art Single-Protoplast Proteomics platform. Utilizing high-resolution mass spectrometry and quantitative proteomics technologies, we achieve high-precision, high-throughput protein identification and quantification from minimal sample amounts. This platform addresses key challenges in single-cell research, such as understanding cellular heterogeneity and deciphering intricate signaling pathways, empowering research in plant biology and other life sciences.

 

 

Our Single-Protoplast Proteomics analysis process is efficient and accurate, comprising:

1. Protoplast Isolation

Mechanical and enzymatic methods are used to isolate protoplasts from plant tissues, ensuring cell membrane integrity.

 

2. Protein Extraction and Quantification

Efficient protein extraction methods are applied to extract proteins from individual protoplasts, followed by precise quantification.

 

3. Digestion and Labeling

Extracted proteins are digested with trypsin, and labeling techniques (e.g., TMT) are used to enhance detection sensitivity across multiple samples.

 

4. Mass Spectrometry Analysis

High-resolution mass spectrometry is employed to analyze the samples, capturing the types and relative abundance of proteins.

 

5. Data Analysis

Advanced bioinformatics tools are used to perform in-depth analysis of the mass spectrometry data, providing protein identification, quantification, and functional annotation.

 



Vu, H.M. et al. J Anal Sci Technol. 2024.

 

Service Advantages

✅ Advanced Mass Spectrometry Platform for Single-Cell Research

We utilize a range of cutting-edge mass spectrometers, such as Orbitrap and Q Exactive, known for their high sensitivity and resolution, which are particularly suitable for proteomics research on single-cell and single-protoplast samples. Our platform is capable of efficiently detecting low-abundance proteins, ensuring comprehensive protein expression profiling even in complex and minute samples.

 

✅ Micro-Sample Handling with Minimal Loss

To address the unique challenges of single-protoplast samples, we have optimized every step of the experimental process, from sample lysis to protein digestion, using specialized techniques to minimize sample loss and maximize the detection of low-abundance proteins. We understand the precious nature of single-protoplast samples and treat each one with the utmost care.

 

✅ Customized Experimental Design

We offer tailored experimental designs based on your research needs, covering everything from sample preparation, selection of labeling and label-free quantification strategies (such as TMT, SILAC, etc.), to the most suitable mass spectrometry analysis approaches. Whether for exploratory experiments or in-depth quantitative analysis, we can provide the optimal solution to help you comprehensively understand proteomic changes in single protoplasts.

 

✅ Comprehensive Data Analysis and Bioinformatics Support

Our bioinformatics team has extensive experience and provides end-to-end analysis services, from raw data processing to biological interpretation. This includes in-depth functional annotation, pathway analysis, statistical validation of differentially expressed proteins, and even the integration of multi-omics data to fully elucidate the biological characteristics of single protoplasts. We are dedicated to transforming complex mass spectrometry data into clear and biologically meaningful conclusions.

 

Sample Results

Exploring the Feasibility of a Single-Protoplast Proteomic Analysis

Recent advances in high-resolution mass spectrometry have enabled the study of proteomes at the single-cell level, offering the potential to reveal new aspects of cellular processes. While single-cell proteomics has been investigated in mammalian cells, no attempts have yet been made to study the proteome of individual plant cells. In this study, we explored the feasibility of applying single-cell proteomics techniques to single protoplast proteomics analysis and identified approximately one thousand proteins from individual protoplasts. As a result, proteins associated with well-known biological processes such as photosynthesis and metabolic pathways were identified.

 



Vu, H.M. et al. J Anal Sci Technol. 2024.

Comparison of Proteins Identified in Single-cell Proteomics Analysis and Bulk Leaf Proteomics Analysis

 

Sample Submission Requirements

1. Sample Type: Plants, fungi, or bacteria

2. Sample Amount: Single cell or small cell numbers (at least 10^3 protoplasts required)

3. Sample Handling: Keep samples at low temperatures to prevent degradation

 

What Could be Included in the Report

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

 

We are committed to delivering precise and efficient solutions through our advanced mass spectrometry platform and high-performance proteomics analysis. Our Single-Protoplast Proteomics Analysis service is designed to support your research by uncovering critical protein dynamics in complex biological processes. Whether you are investigating plant cell differentiation, signal transduction, or host-pathogen interactions, we provide robust data solutions tailored to your needs.

 

Feel free to reach out to us for more detailed information about our services. Let's collaborate and drive cutting-edge discoveries in life sciences together!