Solid-Phase Peptide Synthesis Service

Solid-Phase peptide synthesis is a highly versatile synthetic approach that sequentially attaches amino acids to a solid-phase support (e.g., resin) to construct the desired peptide chain and is widely utilized in biology, drug development, and bioengineering. By overcoming the limitations of traditional liquid-phase synthesis, solid-phase peptide synthesis enables efficient peptide assembly through stepwise coupling and removal of protective groups, offering advantages such as high efficiency, automation, and superior purity. This method effectively addresses challenges like inefficiencies and impurities, making it particularly suited for synthesizing short- to medium-length peptides, including single peptides, functional peptides, and complex polypeptides.

 

MtoZ Biolabs provides tailored solid-phase peptide synthesis service designed to meet the diverse needs of research, development, and production. With advanced technology platforms and a wealth of experience, we ensure the delivery of high-quality peptides, supporting clients in achieving progress in drug discovery, antibody research, and biomarker development.

 

Analysis Workflow

MtoZ Biolabs’ solid-phase peptide synthesis service employs the advanced Fmoc chemistry strategy, ensuring precise and efficient peptide synthesis. The synthesis process involves the following key steps:

1. Resin Loading: The initial amino acid is covalently attached to a functionalized resin, defining the C-terminal properties of the peptide chain.
2. Deprotection: Fmoc protecting groups are removed using a basic reagent, exposing reactive amines for subsequent coupling.
3. Amino Acid Coupling: Activated amino acids are added to the growing peptide chain, forming peptide bonds.
4. Iterative Elongation: The "deprotection-coupling" cycle is repeated until the desired sequence is achieved.
5. Cleavage and Deprotection: Acidic reagents release the peptide from the resin and remove side-chain protective groups.
6. Purification and Quality Control: The peptide is purified using high-performance liquid chromatography (HPLC), and its purity and sequence integrity are verified via mass spectrometry (MS).

 



Ferrazzano, L. et al. Green Chem. 2022.

 

Service Advantages

With a focus on precision and adaptability, our solid-phase peptide synthesis service bridges advanced synthesis technology and stringent quality control to meet diverse research demands, ensuring reliable and high-quality peptides for both routine and complex applications.

• Streamlined Synthesis: Automated workflows significantly reduce production timelines and enhance efficiency.
• High Purity Assurance: Rigorous washing steps at each stage ensure the production of high-purity peptides suitable for downstream applications.
• Customizable Services: Offering tailored synthesis solutions for peptides of varying lengths, structures, and functionalities.
• Complex Peptide Capabilities: Expertise in synthesizing challenging peptides, including those with post-translational modifications or specialized structures.
• Comprehensive Quality Control: Robust quality assurance protocols ensure consistent peptide stability and performance.

 

Applications

Our solid-phase peptide synthesis service enables a wide range of applications, providing reliable and high-quality peptides to advance research in fields such as antibody development, drug discovery, and protein engineering.

• Antibody Research and Vaccine Development: Custom antigenic peptides for antibody screening, immunogenicity studies, and vaccine design.
• Drug Discovery: Active peptides for small molecule screening and target validation.
• Protein Interaction Analysis: Peptide fragments to study protein-protein interactions and functional mechanisms.
• Biomarker Discovery: Tailored peptide biomarkers for disease diagnostics and clinical research.
• Molecular Diagnostics: Peptides for diagnostic reagent development and immunohistochemistry.
• Protein Engineering: Synthetic peptides to investigate protein structure-function relationships.

 

Case Study

Case1

Synthesis of self-assembling peptide-based hydrogels for regenerative medicine using solid-phase peptide synthesis.

 

Pashuck, E. T. et al. Methods Mol. Biol. 2018.

 

 

Case2

High-throughput synthesis of hexapeptides and C-terminal analogs using solid-phase peptide synthesis, revealing the therapeutic potential of Ganoderma-derived peptides in tyrosinase inhibition.

 

 

Krobthong, S. et al. Arch. Pharm. 2021.

 

FAQ

Q1: How is peptide purity ensured in solid-phase peptide synthesis?

At MtoZ Biolabs, peptide purity is rigorously controlled throughout the solid-phase peptide synthesis process. Each synthesis step involves thorough washing to eliminate by-products and unreacted amino acids. HPLC is employed to further purify the final product, ensuring a purity level exceeding 90%, which meets the requirements of most research and development applications. For specialized needs, we also offer ultra-high-purity peptides tailored to specific applications.

 

Q2: Can the quantity of synthesized peptides be customized?

Absolutely. We provide flexible production scales, ranging from small quantities for laboratory use to larger batches for clinical or commercial applications. Our services accommodate diverse project requirements, including preclinical research, pilot studies, and large-scale production.

 

MtoZ Biolabs' solid-phase peptide synthesis service delivers high-quality, high-purity peptides supported by expert customization and technical guidance, driving innovation in research and product development. We look forward to partnering with you to advance the frontiers of biotechnology.