Stable Isotope Labeling Service

Stable isotope labeling is a technique that utilizes stable isotopes to substitute natural isotopes, widely applied for precise molecular tracking and quantitative analysis. By labeling elements such as hydrogen, carbon, and nitrogen in molecules with isotopes, stable isotope labeling assists researchers in elucidating molecular interactions, metabolic pathways, dynamic changes, and their behavior under various conditions.

 

Compared to radioactive isotope labeling, stable isotope labeling offers significant advantages. First, stable isotopes are non-radioactive and pose no radiation risks to researchers or samples, eliminating the need for special safety measures. Second, stable isotopes do not decay over time and can persist for extended periods, making them ideal for long-term tracking and dynamic monitoring. Additionally, stable isotope labeling provides enhanced sensitivity and accuracy, particularly in complex sample analysis, avoiding potential signal interference often associated with radioactive isotopes.

 

Figure 1. The Pprinciple of Stable Isotope Labeling by Amino Acids in Cell Culture

 

As a professional biotechnology service provider, MtoZ Biolabs offers comprehensive Stable Isotope Labeling Service designed to provide researchers and businesses with precise and comprehensive analytical support. Our advanced technological platforms and expert research teams enable us to deliver customized labeling solutions tailored to diverse research needs.

 

Services at MtoZ Biolabs

We offer a range of isotope labeling options, including:

      · ¹³C: Commonly used in metabolic flux analysis, protein labeling, and metabolic pathway research. Especially suited for tracing carbon source transfer and dynamic changes in metabolic pathways.

     · ¹⁵N: Employed in protein synthesis research, quantitative analysis of protein-protein interactions, and nitrogen metabolism studies, allowing precise labeling of nitrogen to reveal its metabolic transformations.

      · ²H: Used in protein quantification and high-precision measurements in mass spectrometry, particularly enhancing accuracy in LC-MS experiments.

      · Other Isotopes: Besides common isotopes like carbon, nitrogen, and deuterium, we offer custom Stable Isotope Iabeling Service with other isotopes such as oxygen-18 and sulfur-34, tailored to specific client needs.

Our Stable Isotope Labeling Service provide not only high-quality isotope-labeled molecules but also integrate advanced mass spectrometry and NMR technologies, offering complete support from sample preparation through data analysis. Whether your research focuses on protein analysis, metabolic studies, drug screening, or toxicology assessments, MtoZ Biolabs delivers precise, reliable experimental data.

 

Why Choose MtoZ Biolabs?

1. Advanced Technology

MtoZ Biolabs utilize cutting-edge labeling technologies and equipment to ensure the highest quality and reliability for every experiment.

 

2. No Radioactive Risk

Our Stable Isotope Labeling Service involve non-radioactive isotopes, eliminating the risk of radioactive contamination and potential health hazards, thus ensuring a safer experimental environment.

 

3. Multidisciplinary Expertise

With experience in proteomics, metabolomics, drug research, and clinical studies, MtoZ Biolabs can address diverse research needs across various fields.

 

4. Tailored Solutions

We provide customized isotope labeling services based on client research needs, ensuring experimental designs and labeling strategies best align with your research objectives.

 

5. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

Applications

Stable Isotope Labeling Service finds widespread applications across various research domains, including, but not limited to:

• Proteomics: Precise quantification of protein expression levels, analysis of protein-protein interactions, and examination of post-translational modifications.
• Metabolomics: Investigation of dynamic changes in intracellular and extracellular metabolites.
• Drug Development: Application in drug metabolism, pharmacokinetics, and pharmacodynamics research, aiding in the development of novel drugs and the evaluation of their bioavailability.
• Biomarker Research: Facilitating the identification of potential disease biomarkers, thereby advancing the application of precision medicine.

 

Case Study

Case 1: Application of Stable Isotope Labeling in Mouse T Cell Metabolism Analysis

This study utilized stable isotope labeling in combination with rapid cell sorting to analyze the metabolic processes of mouse T cells in vivo. By introducing stable isotopes, the researchers were able to precisely track the metabolic dynamics of T cells in different immune states and uncover the mechanisms underlying metabolic reprogramming. The enhanced sensitivity of metabolite quantification and the elucidation of metabolic pathways underscored the powerful advantages of Stable Isotope Labeling Service in cell metabolism research, particularly in immunology and cancer immunotherapy.

 



Sheldon, R. D. et al. Nat. Protoc. 2021.

 

Case 2: Application of Stable Isotope Labeling in Molecular Quantification of Brain Tissue

In this study, researchers employed stable isotope-labeled mRNA cap structures synthesized via the P(III)-Amidite strategy for high-sensitivity quantitative analysis in brain tissue. The use of stable isotopes significantly improved the precision and sensitivity of molecular quantification, especially in complex biological samples such as brain tissue. The stable isotope labeling enhanced the reliability and resolution of molecular detection, demonstrating its crucial role in the accurate quantification of biomarkers in neuroscience and neurobiology research.

 



Ripp, A. et al. Angew. Chem. Int. Ed. 2024. 

 

FAQ

Q1: Does Stable Isotope Labeling Affect Molecular Structure or Function?

The effect of stable isotope labeling on molecular structure and function is typically negligible because the chemical properties of stable isotopes closely resemble those of their natural counterparts. During labeling, the introduced isotopes only replace certain elements in the molecule, generally without altering its chemical reactivity or biological activity. Thus, stable isotope labeling can be used with confidence for high-precision molecular analysis.

 

Q2: How Can I Determine if an Isotope is Stable?

The stability of an isotope is determined based on the atomic nucleus. Stable isotopes are characterized by a balanced proton-to-neutron ratio in their atomic nuclei, which prevents radioactive decay. Researchers often assess isotope stability by examining its half-life—longer half-lives typically indicate greater stability. Additionally, experimental data and theoretical models aid in confirming the stability of isotopes.

 

Q3: What Types of Molecules Can I Label?

At MtoZ Biolabs, we offer customized Stable Isotope Labeling Service for a variety of compounds. Common sample types include, but are not limited to: NMR solvents, specialty gases, amino acids, cell culture media, nucleic acids/nucleotides, drugs/metabolites, fatty acids/lipids, and carbohydrates. We provide flexible labeling solutions tailored to your specific research needs, ensuring labeling accuracy and experimental reliability.

 

MtoZ Biolabs is committed to providing high-quality Stable Isotope Labeling Service to support your research breakthroughs in precision and depth. If you have any questions or need further consultation, feel free to contact our professional team at any time.