SWATH Mass Spec Service

SWATH Mass Spec Service enables the quantitative analysis of thousands of proteins simultaneously in a single experiment, providing comprehensive quantitative information on proteins. It is effectively utilized in areas such as disease biomarker discovery, protein expression change analysis, drug development, and biomarker screening. This technology is particularly well-suited for large-scale sample analyses and the identification and quantification of proteins in complex biological samples.

 



Krasny, L. et al. J Proteomics. 2018.

Swath Mass Spectrometry as a Tool for Quantitative Profiling of the Matrisome

 

SWATH (Sequential Window Acquisition of All Theoretical Ions) mass spectrometry technology is a quantitative analysis method based on high-resolution mass spectrometers, designed to enhance the comprehensiveness and accuracy of data acquisition through a full-spectrum acquisition strategy. The core principle involves dividing the mass spectrometry range into multiple predefined small windows during high-resolution mass spectrometry analysis and performing comprehensive ion collection within each window. Unlike traditional mass spectrometry methods such as Data-Dependent Acquisition (DDA), SWATH employs a "full ion acquisition" approach, scanning all theoretically possible fragment ions within each window and retaining all ion information for subsequent analysis. This eliminates the data loss issues inherent in DDA methods.

 

As a result, SWATH Mass Spec Service offers high quantitative precision and achieves high-throughput, whole-protein quantification without the need for ion-selective methods. It is especially suitable for proteomic analyses of complex samples. In the post-mass spectrometry data analysis phase, combining database searching with spectral matching techniques, SWATH Mass Spec Service can achieve precise protein quantification and reveal changes in the relative abundance of proteins. It is widely applied in fields such as biomarker discovery, disease mechanism research, and drug development.

 

Service at MtoZ Biolabs

MtoZ Biolabs' SWATH Mass Spec Service utilizes industry-leading mass spectrometry technology to provide high-precision, high-throughput proteomic analysis solutions. Through SWATH technology, MtoZ Biolabs can simultaneously capture quantitative information for thousands of proteins in a single experiment, deeply analyzing protein expression, post-translational modifications, interactions, and functional networks within complex biological samples. This service is particularly suitable for disease biomarker discovery, drug development, and clinical research, significantly enhancing data reproducibility and reliability while advancing precision medicine and biomarker research.

 



 

Service Advantages

✅ High-Throughput Analysis

By analyzing thousands of proteins simultaneously, the SWATH Mass Spec Service greatly increases the throughput of proteomic research and provides highly reproducible data. This makes SWATH Mass Spec an ideal choice for large-scale screening, biomarker discovery, and multi-omics studies.

 

✅ Full-Spectrum Data Acquisition

Unlike traditional mass spectrometry methods, SWATH captures all fragment ion data across the entire mass spectrometry range, enabling comprehensive quantification of target proteins and their isoforms. This full-spectrum acquisition ensures precise detection of changes in protein expression and post-translational modifications.

 

✅ High Sensitivity and Reproducibility

The SWATH Mass Spec Service can detect low-abundance proteins in complex samples and offers a wide dynamic range, effectively distinguishing signals from both low- and high-abundance proteins. This ensures comprehensive and accurate protein quantification.

 

✅ Unbiased Data Acquisition

SWATH’s full-spectrum acquisition mode avoids the selective ion monitoring (SIM) that relies on prior knowledge in traditional mass spectrometry methods, thereby preventing analytical biases caused by incorrect target selection. This makes SWATH an ideal tool for discovering unknown biomarkers and exploring new protein functions.

 

Applications

✅ Protein Expression Profiling

✅ Large-Scale Protein Quantification

✅ Drug Target Identification and Screening

✅ Disease Biomarker Discovery

✅ Disease Pathology and Genetic Association Studies

 

Case Study

Scanning SWATH Mass Spec Achieves Ultra-Fast, High-Precision Proteomic Quantitative Analysis

Proteomic research requires high-throughput and high-precision quantitative analysis techniques. SWATH Mass Spec, as a data-independent acquisition (DIA) technology, can simultaneously analyze multiple proteins, offering high coverage and stable quantification capabilities. However, traditional SWATH Mass Spec employs fixed window scanning, resulting in lower acquisition speeds and data decoding efficiencies, which limits its application scope. The literature introduces Scanning SWATH Mass Spec, which replaces fixed window scanning with dynamically adjusted ion acquisition windows. By combining high-speed scanning with advanced spectral library decoding algorithms, it optimizes acquisition efficiency and signal-to-noise ratio. Additionally, this method incorporates improved models in data processing, significantly reducing data decoding time while enhancing the precision and depth of quantification.

 

The results demonstrate that Scanning SWATH Mass Spec significantly improves the speed and coverage of proteomic quantitative analysis. In experiments, this technology exhibited higher sensitivity, better signal-to-noise ratios, and deeper analytical coverage. Compared to traditional SWATH Mass Spec, it can achieve high-precision analysis of complex samples in a shorter time frame.

 



Messner, C. B. et al. Nat Biotechnol. 2021.

Minute Gradients and Scanning SWATH Identify Biomarkers that Classify Covid-19 Patients

 

MtoZ Biolabs' SWATH Mass Spec Service is dedicated to providing clients with cutting-edge proteomics analysis solutions. Our service combines highly sensitive mass spectrometry platforms with advanced data processing technologies, enabling researchers to extract precise quantitative data from complex biological samples. This supports multiple fields, including large-scale protein analysis, disease biomarker discovery, and mechanism of action studies. Based on efficient, reliable, and customized services, we welcome collaborations with global research institutions, pharmaceutical companies, and biotechnology firms to jointly advance the depth of biomedical research.